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不孕不育症与支原体感染关系探讨
Detection for Ureaplasma Urealyticum in Infertile Women and Men
【摘要】 目的:探讨不孕不育症与溶脲脲原体(UU)感染之间的关系,建立检测UU的实验方法。方法:采用培养法、聚合酶链反应(PCR)和ABC法分别对78例不孕妇女和61例不育男性患者进行UU检测,并将结果进行比较。选择有孕育史的39例女性和30例男性作为对照。结果:不孕不育组UU阳性检出率明显高于对照组(P<0.05);ABC法对临床标本的检出率与培养法和PCR法比较,在统计学上差异无显著性(P>0.05)。结论:女性不孕和男性不育均与UU感染密切相关,ABC法可作为诊断UU感染的快速检测方法。
【Abstract】 Objective:To elucidate the association of ureaplasma urealyticum(UU) infection and infertile disease in women and men.And set up the fast immune method in detecting UU.Methods:UU were examined respectively in the secretion by cultivation and PCR technique in 78 infertile women and 61 infertile men assigned as monitored groups,69 women and men of normal procreation assigned as control groups.Clinical specimens were also examined by avidin biotimperoxidase complex technique(ABC technique).The positive rates were then compared the monitored groups with the control groups.Results:There were significant differences in the positive rate of UU between monitored groups(33.09%) and control groups(10.14%);ABC technique positive specimens were found among 51 cases,there were no statistical significant among three methods(P>0.05).Conclusion:It is suggested that infertility in women and men was closely correlated with UU infection.ABC technique was simple and fast,and was of clinical significance in diagnosis of UU infection.
【Key words】 Infertility; Ureaplasma urealyticum; Immunoenzymatic technique; Nongonococcal urethitis;
- 【文献出处】 中国妇幼保健 ,Maternal and Child Health Care of China , 编辑部邮箱 ,2006年11期
- 【分类号】R711.6
- 【被引频次】4
- 【下载频次】84