【摘要】 目的观察褪黑素对体外缺氧缺糖SHSY 5Y细胞的保护作用及线粒体通透性转换孔开放对该保护作用的影响。方法体外培养SHSY 5Y神经母细胞瘤株,以缺氧缺糖(OGD)、褪黑素(10μm o l/L)伴或不伴苍术苷(atracty los ide)(20μm o l/L)处理,以M TT检测SHSY 5Y细胞活性,应用ca lce in-AM、HE t和fluo-3/AM的荧光强度测定OGD后SHSY 5Y细胞线粒体通透性转换孔(M TPT)开放、细胞内超氧阴离子和钙离子的浓度。结果褪黑素能提高OGD后SHSY 5Y细胞的活性,这种神经保护作用可被苍术苷阻断。褪黑素降低OGD后ca lce in-AM、HE t和fluo-3/AM的荧光强度。结论褪黑素通过抑制SHSY 5Y细胞M TPT开放,来降低细胞内超氧阴离子的浓度,进而抑制细胞内钙超载,从而达到保护SHSY 5Y细胞对抗OGD的细胞损害的作用。更多还原

【Abstract】 Objective To investigate the neuroprotective effect of melatonin on SHSY5Y neuronal cells against oxygen-glucose deprivation(OGD),and the role of the opening of the mitochondrial permeability transition pore(MPTP)in the effect.Methods SHSY5Y neuroblastoma cells were exposed to OGD,melatonin(10μM)alone or melatonin together with atractyloside(20 μmol/L).Cell viability was measured by the MTT assay,opening of MPTP,intracellular concentration of superoxide anion and calcium were evaluated via the fluorescence intensity of calcein-AM,HEt and fluo-3/AM.Results After OGD,compared with the vehicle group,the MTT value was elevated significantly in the melatonin group(P<(0.01)).The neuroprotective effect of melatonin was blocked by administration of atractyloside.The fluorescence intensity of calcein-AM(P<(0.05)),HEt(P<(0.05))and fluo-3/AM(P<(0.01))was decreased significantly in the melatonin group.Conclusion Melatonin protects SHSY5Y neuronal cells against OGD by inhibiting the opening of MPTP and reducing the intracellular concentration of superoxide anion and calcium.更多还原