【摘要】 目的建立雷公藤及其片剂中雷公藤对醌B测定的HPLC法。方法采用外标法。H iQ s iL KYA-C18(250mm×4.6 mm,5μm)色谱柱为固定相,甲醇-1%冰醋酸水溶液(66∶34)为流动相,检测波长为254 nm,体积流量为1.0 mL/m in。结果线性范围为6.62~105.9μg/mL(r=0.999 9),湖南产雷公藤平均回收率为97.78%,RSD为1.09%(n=9),昆明山海棠片平均回收率为99.83%,RSD为1.76%(n=9)。结论本方法准确、灵敏,可作为雷公藤类药材及其片剂中雷公藤对醌B的定量分析方法。更多还原

【Abstract】 Objective To establish an HPLC method for determination of triptoquinone B in Radix Folium Seu Flos Tripterygii Wilfordii（RFFTW） and its tablets.Methods An external method with HiQ siL KYA-C₁₈（250 mm×4.6 mm,5 μm） column as fixed phase and methanol-1% acetic acid solution（（66∶）34） as mobile phase was adopted.The detective wave length was 254 nm and the flow rate was 1.0 mL/min.Results The linearity range was 6.62—105.9 μg/mL（r=0.999 9）,the average recovery of T.wilfordii from Hunan Province was 97.78%,RSD was 1.09%（n=9）,and the average recovery of tablet of T.hypoglaucum was 99.83%,RSD was 1.76%（n=9）.Conclusion The method is accurate and sensitive.It is adoptable for quantitative analysis of triptoquinone B in RFFTW and its tablets.更多还原