【摘要】 目的从DNA水平分析鉴定不同薯蓣皂苷元盾叶薯蓣的遗传关系。方法从40个10 bp随机引物中筛选出12个引物,进行RAPD分析,应用POPGENE 1.31软件对扩增结果进行聚类分析。结果共扩增出73条DNA片段,其中多态性片段64条,占87.67%,盾叶薯蓣类型间具有明显的多态性差异。结论DNA分子多样性差异与薯蓣皂苷元量差异具有相关性,说明盾叶薯蓣的遗传基础可能对薯蓣皂苷元的形成和积累有显著的影响。更多还原

【Abstract】 Objective To study the genetic relationship among Dioscorea zingiberensis with different diosgenin content at DNA level.Methods DNA of all types was amplified by 12 random primers from 40 random primers,the polymorphic bands of RAPD were counted.And the results of types were analysed by POPGENE 1.31(Tool for Population Genetic Analysis 1.31) software.Results A total of 73 bands was obtained,64 of total bands(87.67%) was polymorphic.The results indicated that there were apparent and abundant genetic variations in D.zingiberensis. Conclusion The formation and accumulation of diosgenin could be affected significantly by the genetic basis of D.zingiberensis.更多还原