【摘要】 目的:建立测定五参芪口服液(人参,黄芪,女贞子等)中人参皂苷Rg1和人参皂苷Re含量的反相高效液相色谱分析方法。方法:色谱柱:Hypersil-C18(5μm,200 mm×4.6 mm I.D),柱温30℃,流动相为乙腈-0.05%磷酸水溶液(103∶400),检测波长203 nm,流速1.0 mL/m in。结果:人参皂苷Rg1和人参皂苷Re分别在0.188~6.016μg(r=0.999 9)和0.197 6~6.323 2μg(r=0.999 9)范围内线性关系良好。平均回收率分别为99.53%和99.13%。RSD分别为0.96%和0.62%。结论:本法操作简便、结果准确,重现性好,可用于五参芪口服液中人参皂苷Rg1和人参皂苷Re的含量测定。更多还原

【Abstract】 AIM: To develop a RP-HPLC method of determining ginsenoside Rg1 and ginsenoside Re in Wu-（shenqi） Oral Liquid（Radix et Rhizoma Ginseng,Radix Astragali,Fructus Ligustri Lucidi,etc.）. METHODS: Hypersil-C₁₈ column was used at 30 ℃.The mobile phase consisted of acetonitrile-water（103∶400） with 0.05% H₃PO₄.The detection wavelength was set at 203 nm.The flow rate was 1.0 mL/min. RESULTS: The linear ranges for ginsenoside Rg1 and ginsenoside Re were 0.188-6.016 μg（r=0.999 9） and 0.197-6.323 μg（r=（0.999））,respectively.Its average recoveries were 99.53% with RSD of 0.96% and 99.13% with RSD of 0.62%,respectively. CONCLUSION: This method is simple,accurate,reproducible,and can be used for the determination of ginsenoside Rg1 and ginsenoside Re in Wushenqi Oral Liquid.更多还原