【摘要】 目的:研究HPD-600大孔树脂纯化山楂叶总黄酮的工艺条件及参数。方法:采用正交实验法,以固形物中总黄酮含量为考察指标,对影响黄酮提取工艺的因素进行了研究。结果:在实验设计的影响因素中,上柱粗提液浓度和洗脱液组成对纯化效果有显著影响。结论:经微波辅助提取并通过大孔吸附树脂纯化后,山楂叶产品中总黄酮含量可达80.75,芦丁含量达1.38,说明本法纯化山楂黄酮是可行的。更多还原

【Abstract】 AIM: To study the technological parameters of the purification process of total flavonoids of Crataegus leaves with HPD-600 macroreticular resin. METHODS: Using orthogonal experiments,the adsorptive characteristics and elutive parameters of the process were studied by taking the elutive effects and purification ratio of total flavonoids of Crataegus leaves. RESULTS: Two significant factors which were the concentration of crude flavonoids solvent and elutant component were observed. CONCLUSION: The microwave-assisted extraction and purification process with macroreticular resin is employed,the content of total flavonoids is 80.75%, the rutin content is (1.38)%, The macroreticular resin can be used in the purification of flavonoids of Crataegus leaves.更多还原