【摘要】 目的:观察糖尿病大鼠肝过氧化物酶体脂肪酸β-氧化的变化。方法:以链脲佐菌素(STZ)制备糖尿病大鼠模型,通过透射电镜观察肝脏过氧化物酶体数量和形态学的改变,采用分光光度法和荧光光度法分析参与过氧化物酶体脂肪酸β-氧化的多种酶活性的改变。结果:糖尿病大鼠血清甘油三酯高于对照组,高密度脂蛋白胆固醇低于对照组;肝脏过氧化物酶体数量多于对照组,脂酰CoA氧化酶、过氧化氢酶和过氧化物酶体脂肪酸β-氧化活性显著大于对照组。结论:糖尿病大鼠肝过氧化物酶体脂肪酸β-氧化增强,与糖尿病状态下脂质代谢紊乱有关。更多还原

【Abstract】 AIM: To clarify the change of peroxisomal fatty acid β-oxidation in diabetic rats and its correlation to lipid metabolism disorder in diabetes mellitus.METHODS: Diabetic rat model was induced by streptozotocin(STZ) injection.The number and morphological changes of peroxisomes were compared by electronic microscope.The activities of major enzymes involved in peroxisomal β-oxidation were detected by spectrophotometric and fluorometric assay in livers from both STZ-induced diabetic and control rats.RESULTS: The level of serum triglyceride was significantly higher and the level of serum high-density lipoproteir cholesterol was significantly lower in STZ-induced diabetic rats than those in control rats.In the liver of diabetic rats,the peroxisomes proliferated and fatty acid β-oxidation of peroxisome was strengthened.The activities of catalase and fatty acyl-CoA oxidase in the diabetic rat liver were significantly increased compared to control rats.CONCLUSION: In diabetic state,lipid metabolism disorder continuously exists.The peroxisomal fatty acid β-oxidation strengthens and the activities of major enzymes involved in peroxisomal β-oxidation are significantly increased in the liver of STZ-induced diabetic rat.更多还原