节点文献

S-甲基异硫脲对阿霉素致大鼠心肌组织超氧化物歧化酶、谷胱甘肽过氧化物酶活性改变的影响

Effect of S-methylisothiourea on activities of superoxide dismutase and glutathione peroxidase in myocardial tissues of rats treated with adriamycin

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 阳冠明孙胜涛李树全林伟雄叶司原利基林林善修

【Author】 YANG Guan-ming~1,SUN Sheng-tao~1,LI Shu-quan~1,LIN Wei-xiong~1,YE Si-yuan~2,LI Ji-lin~2,LIN Shan-xiu~1(~1 Department of Pediatrics,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;~2 Department of Biochemistry,The Guangxi Cancer Institute,Nanning 530021, China)

【机构】 广西医科大学第一附属医院儿科广西肿瘤研究所生化室广西医科大学第一附属医院儿科 广西南宁530021广西南宁530021

【摘要】 目的:探讨S-甲基异硫脲(SMT)对阿霉素(ADM)所致大鼠心肌组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)活性改变的影响。方法:给大鼠腹腔注射ADM(10.0 mg.kg-1)1次,给ADM处理的大鼠静脉注射不同剂量的SMT(每天1次,共3次)进行干预。用分光光度法测定心肌组织丙二醛(MDA)含量、NO2-/NO3-含量、锰超氧化物歧化酶(MnSOD)活性、铜-锌超氧化物歧化酶(Cu-ZnSOD)活性、谷胱甘肽过氧化物酶(GPx)活性、一氧化氮合酶活性;用酶的速率法测定血清肌酸激酶(CK)的同功酶CK-MB活性;用逆转录-聚合酶链反应方法检测心肌组织MnSOD mRNA、Cu-ZnSOD mRNA、GPx mRNA、诱导型一氧化氮合酶(iNOS)mR-NA表达。结果:SMT(5.0,10.0,20.0 mg.kg-1)3个干预组心肌组织MDA含量、NO2-/NO3-含量、iNOS活性及血清CK-MB活性明显低于ADM组(P<0.01),而其心肌组织MnSOD mRNA、Cu-ZnSOD mRNA、GPx mRNA表达水平及其酶活性明显高于ADM组(P<0.01)。结论:SMT拮抗ADM抑制心肌组织MnSOD mRNA、Cu-ZnSODmRNA、GPx mRNA表达而拮抗ADM抑制这些酶的活性。

【Abstract】 AIM: To study the effect of S-methylisothiourea(SMT) on adriamycin(ADM)-induced changes of superoxide dismutase and alutathione peroxidase activities in myocardial tissue of rats.METHODS: Rats were treated with ADM by intraperioneal injection (10.0 mg/kg body weight),and then the ADM-treated rats were intervened by SMT at different dosages by intravenous injection(once a day for three days).The contents of malondialdehyde(MDA) and NO2-/ NO3-,the activities of manganes superoxied dismutase(MnSOD),copper-zinc superoxide dismutase(Cu-Zn SOD),glutathione peroxidase(GPx) and inducible nitric oxide synthase(iNOS) were determined by spectrophotometry in myocardial tissue.The activity of isoenzymic CK-MB of creatine kinase(CK) was determined by enzymic rate method in serum.The expression of MnSOD mRNA,Cu-ZnSOD mRNA,GPx mRNA and iNOS mRNA were detected by reverse transcription-ploymerase chain reaction.RESULTS: The contents of MDA,NO-2/ NO-3,and iNOS activity of myocardial tissue,the activity of CK-MB in serum in SMT(5.0,10.0,20.0 mg/kg) intervention groups were significantly lower than those in ADM-treated group(P<0.01).However,the activities of MnSOD,Cu-ZnSOD and GPx in myocardial tissue and levels of mRNA expression were significantly higher than those in ADM-treated group(P<0.01).CONCLUSION: SMT antagonizes the inhibitory effects of ADM on the expression of MnSOD mRNA,Cu-ZnSOD mRNA and GPx mRNA in myocardial tissue,there by antagonizes the inhibitory effects of ADM on the activities of MnSOD,(CuZnSOD) and GPx in myocardial tissue.

【基金】 广西自然科学基金资助项目(No.0447050)
  • 【文献出处】 中国病理生理杂志 ,Chinese Journal of Pathophysiology , 编辑部邮箱 ,2006年12期
  • 【分类号】R96
  • 【被引频次】3
  • 【下载频次】180
节点文献中: 

本文链接的文献网络图示:

本文的引文网络