【摘要】 目的:探讨S-甲基异硫脲(SMT)对阿霉素(ADM)所致大鼠心肌组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)活性改变的影响。方法:给大鼠腹腔注射ADM(10.0 mg.kg-1)1次,给ADM处理的大鼠静脉注射不同剂量的SMT(每天1次,共3次)进行干预。用分光光度法测定心肌组织丙二醛(MDA)含量、NO2-/NO3-含量、锰超氧化物歧化酶(MnSOD)活性、铜-锌超氧化物歧化酶(Cu-ZnSOD)活性、谷胱甘肽过氧化物酶(GPx)活性、一氧化氮合酶活性;用酶的速率法测定血清肌酸激酶(CK)的同功酶CK-MB活性;用逆转录-聚合酶链反应方法检测心肌组织MnSOD mRNA、Cu-ZnSOD mRNA、GPx mRNA、诱导型一氧化氮合酶(iNOS)mR-NA表达。结果:SMT(5.0,10.0,20.0 mg.kg-1)3个干预组心肌组织MDA含量、NO2-/NO3-含量、iNOS活性及血清CK-MB活性明显低于ADM组(P<0.01),而其心肌组织MnSOD mRNA、Cu-ZnSOD mRNA、GPx mRNA表达水平及其酶活性明显高于ADM组(P<0.01)。结论:SMT拮抗ADM抑制心肌组织MnSOD mRNA、Cu-ZnSODmRNA、GPx mRNA表达而拮抗ADM抑制这些酶的活性。更多还原

【Abstract】 AIM: To study the effect of S-methylisothiourea（SMT） on adriamycin（ADM）-induced changes of superoxide dismutase and alutathione peroxidase activities in myocardial tissue of rats.METHODS: Rats were treated with ADM by intraperioneal injection （10.0 mg/kg body weight）,and then the ADM-treated rats were intervened by SMT at different dosages by intravenous injection（once a day for three days）.The contents of malondialdehyde（MDA） and NO₂^-/ NO₃^-,the activities of manganes superoxied dismutase（MnSOD）,copper-zinc superoxide dismutase（Cu-Zn SOD）,glutathione peroxidase（GPx） and inducible nitric oxide synthase（iNOS） were determined by spectrophotometry in myocardial tissue.The activity of isoenzymic CK-MB of creatine kinase（CK） was determined by enzymic rate method in serum.The expression of MnSOD mRNA,Cu-ZnSOD mRNA,GPx mRNA and iNOS mRNA were detected by reverse transcription-ploymerase chain reaction.RESULTS: The contents of MDA,NO^-₂/ NO^-₃,and iNOS activity of myocardial tissue,the activity of CK-MB in serum in SMT（5.0,10.0,20.0 mg/kg） intervention groups were significantly lower than those in ADM-treated group（P<0.01）.However,the activities of MnSOD,Cu-ZnSOD and GPx in myocardial tissue and levels of mRNA expression were significantly higher than those in ADM-treated group（P<0.01）.CONCLUSION: SMT antagonizes the inhibitory effects of ADM on the expression of MnSOD mRNA,Cu-ZnSOD mRNA and GPx mRNA in myocardial tissue,there by antagonizes the inhibitory effects of ADM on the activities of MnSOD,（CuZnSOD） and GPx in myocardial tissue.更多还原