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H₂O₂对大鼠肺动脉平滑肌细胞Kv通道的作用

Effect of H₂O₂ on voltage-gated potassium channels in rat pulmonary artery smooth muscle cells

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【作者】骆红艳；唐明；杜以梅；刘长金；唐碧；胡新武；胡谋先；席姣娅；宋元龙； Jurgen Hescheler；

【Author】 LUO Hong-yan~1,TANG Ming~1,DU Yi-mei~1,LIU Chang-jin~1,TANG Bi~1,HU Xin-wu~1,HU Mou-xian~1,XI Jiao-ya~1,SONG Yuan-long~1,Jurgen Hescheler~2(~1Department of Physiology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China.com;~2 Institute of Neurophysiology,University of Cologne,D-50931 Cologne,Germany)

【机构】华中科技大学同济医学院生理系； Institute of Neurophysiology University of Cologne； D-50931 Cologne； Germany；湖北武汉430030；

【摘要】目的:观察H2O2在常氧时对大鼠肺动脉平滑肌细胞(PASMCs)的Kv的影响,探讨H2O2对肺动脉平滑肌细胞的Kv通道的作用。方法:用酶解法急性分离单个PASMCs,以全细胞膜片钳技术记录PASMCs膜上的电压依赖性钾通道(voltage-gated potassium channel,Kv)电流。结果:常氧下H2O2可显著增加Kv电流,电流-电压关系曲线左上移;而且Kv电流呈浓度依赖性增加。结论:常氧下H2O2可使Kv通道开放。更多还原

【Abstract】 AIM: To determine the effect of hydrogen peroxide（H₂O₂） on voltage-gated potassium channel currents（IKv） in pulmonary vascular smooth muscle cells（PASMCs）.METHODS: Using whole cell patch-clamp technique,IKv was recorded in freshly isolated rat PASMCs with acute enzymatic digestion method.The effect of hydrogen peroxide on IKv in PASMCs was investigated in normoxia.RESULTS: IKv in PASMCs was increased significantly by H₂O₂ and the increase depended on the concentration in normoxia.Current-voltage relationship curve shifted to the left.CONCLUSION: Hydrogen peroxide is an important K⁺ channel opener.更多还原