【摘要】 目的:观察干扰素-α(IFN-α)对人胃癌细胞株BGC-823生长和浸润转移的抑制作用及其作用机理。方法:在体外培养体系中检测IFN-α对BGC-823细胞株生长增殖能力的影响,MTT法检测细胞活力,流式细胞仪检测细胞周期。同时采用免疫组化法观察了IFN-α对肿瘤细胞E-cadherin、MMP-2表达的调节作用,电镜下观察肿瘤细胞间连接的超微结构的变化。结果:IFN-α对人胃癌细胞株BGC-823的生长有明显抑制作用,并表现出剂量依赖性,IFN-α浓度≥106U/L时可有效抑制细胞增殖,抑制率≥12.2%,细胞周期在G1-S期之间发生阻滞;在IFN-α诱导下细胞E-cadherin表达水平升高而MMP-2表达下降,超微结构出现了细胞粘附连接数量增多、结构相对紧密等变化。结论:IFN-α可通过影响细胞周期来抑制人胃癌细胞株BGC-823的生长;IFN-α能调节E-cadherin、MMP-2的表达,密切细胞间的连接,具有限制胃癌细胞侵袭转移的潜能。更多还原

【Abstract】 AIM:To observe the inhibitory effect of interferon-α（IFN-α）on the growth invasiveness and metastasis of human gastric carcinoma cell line BGC-823,and mechanism of its action.METHODS:We detected the influence of IFN-α on the proliferative ability of BGC-823 in cell culture system,the cell vitality with the MTT colorimetric assay,and the cell cycle with flow cytometer（FCM）.The regulatory functions of IFN-α to the expression of E-cadherin and matrix metalloproteinase-2（MMP-2）in tumor cells were estimated by immunohistochemical analysis（S-P）.The ultrastructural changes of the junction among the tumor cells were observed under electron microscope.RESULTS:IFN-α can significantly inhibit the growth of human gastric carcinoma cell line BGC-823 in a dose-dependent manner.When the concentration of IFN-α was ≥106 U/L,the cell proliferation can be effectively suppressed,the suppression rate was ≥12.2%,and the blockage appeared at the phase of G₁-S of the cell cycle.Under the induction of IFN-α,the expression level of the cell E-cadherin increased while the MMP-2 decreased.The changes on ultrastructure of the cells showed the increased adhesive junctions and the relative compact structure.CONCLUSION:IFN-α can suppress the growth of human gastric carcinoma cell line BGC-823 through its influence on cell cycle.IFN-α can regulate the expression of E-cadherin and MMP-2,make the cell junction closely,so that it has the potential on restricting the invasion and metastasis of gastric carcinoma cells.更多还原