【摘要】 目的:研究[G ly14]-hum an in对β淀粉样蛋白1-42引起的神经干细胞毒性的作用。方法:用[G ly14]-hum an in和β淀粉样蛋白1-42处理神经干细胞,观察其对神经干细胞增殖、分化的影响。结果:较低浓度的[G ly14]-hum an in加入有β淀粉样蛋白1-42处理的神经干细胞培养基,经过琼脂糖凝胶电泳分析及流式细胞测定DNA含量,神经干细胞显示出对β淀粉样蛋白1-42的耐受,而对照组则显示神经干细胞出现凋亡现象。高浓度的[G ly14]-hum an in加入培养基,经台盼蓝拒染法计数细胞,神经干细胞死亡率明显低于对照组,对照组神经干细胞出现大量死亡。结论:[G ly14]-hum an in不但具有抑制β淀粉样蛋白1-42对神经干细胞的毒性作用,而且在低浓度的情况下,可以促进神经干细胞的增殖和分化为神经元。更多还原

【Abstract】 AIM: To study the effects of [Gly¹⁴]-humanin,one of the strongest derivate of humanin,on proliferation and differentiation of neural stem cells（NSCs） and the protective action of cell death or apoptosis induced by β-amyloid protein 1-42.METHODS: NSCs were treated with different concentrations of [Gly¹⁴]-humanin and β-amyloid protein 1-42.RESULTS: 10 nmol/L [Gly¹⁴]-humanin made NSCs resistant to the apoptotic action induced by AβP1-42 and prevented NSCs from death induced by 25 μmol/L β-amyloid protein 1-42.The differentiated neural stem cells yield more neuronal cells than that in control groups when 10 nmol/L [Gly¹⁴]-humanin was added to the culture media.The number of cells increased and the cultures grown with a manner of floating cell clones likes that cultured in the presence of mitogen when 100 nmol/L [Gly¹⁴]-humanin was added to the differentiation culture media.CONCLUSION: The [Gly¹⁴]-humanin significantly promoted the proliferation and neuronal differentiation of neural stem/progenitor cells and also inhibited the toxic action of β-amyloid protein 1-42 on cultured neural stem/progenitor cells.更多还原