【摘要】 目的:研究不同剂量左旋多巴对偏侧帕金森病大鼠行为学及多巴胺D2受体表达数的影响,并对其相应机制进行探讨。方法:采用6-羟多巴胺(6-OHDA)制备偏侧PD大鼠模型,用免疫组化染色法检测PD模型大鼠分别给予左旋多巴不同剂量(10 mg.kg-1.d-1,50 mg.kg-1.d-1,100 mg.kg-1.d-1)腹腔注射15 d前后纹状体部位的多巴胺D2受体表达数、观察大鼠行为学改变。结果:成功PD模型大鼠30 m in旋转圈数与损毁侧及其健侧的D2受体表达数增加百分数呈线性相关(r=0.927,P<0.01),大剂量左旋多巴干预后PD大鼠的旋转圈数明显少于对照组(P<0.05),且损毁侧纹状体多巴胺D2受体表达数明显少于对照组(P<0.01)。结论:大剂量左旋多巴干预能明显改善PD大鼠的旋转行为,并使纹状体多巴胺D2受体明显下调。更多还原

【Abstract】 AIM: To observe the effects of different doses of L-dopa on the rotational behavior and amounts of cells expressing D₂ receptors in striatum in hemiparkinsonian rats.METHODS: A hemiparkinsonian model was established in rats by pretreatment with 6-hydroxydopamine.The D₂ receptor expression were detected by immunohistochemical staining.The numbers of rotations induced by apomorphine was counted within 30 min before and after L-dopa(10 mg·kg^-1·d^-1,50 mg·kg^-1·d^-1 or 100 mg·kg^-1·d^-1,ip) was introduced to Parkinson’s disease（PD） model rats for 15 days.RESULTS: In successful PD model rats,the increased percentage of D₂ receptor in lesioned side compared with intact side was associated linearly with the numbers of rotations within 30 min（r=0.927,P<0.01）.After high dose of（L-dopa） intervention to PD model,the numbers of rotations decreased significantly（P<0.05）,the（amounts） of cells expressing D₂ receptor at the lesioned side striatum decreased significantly（P<0.01）.CONCLUSION: After high dose of L-dopa intervention,rotation behavior of PD rats improves,and D₂ receptor is down-regulated significantly.更多还原