【摘要】 目的:观察多巴胺受体(DR)1和2mRNA和蛋白质在大鼠病理性心肌肥厚时的表达情况。方法:应用肾动脉缩窄术复制Wistar大鼠心肌肥厚的动物模型。于术后35d取心脏,测定心肌肥大指数,左室内压,V-G染色观察胶原含量。应用心肌原位杂交和RT-PCR,免疫荧光,Westernblotting结合图像分析系统分别检测心肌组织中多巴胺受体D1、D2mRNA和蛋白质的表达变化。结果:DR1、DR2mRNA在正常大鼠心肌组织有表达,其中血管平滑肌细胞内DR2的分布多于心室肌和心房肌细胞。模型组左心肥大明显,表现为室内压显著升高,胶原含量增多;模型组心室肌DR1和DR2mRNA和蛋白质的含量均明显低于假手术组。结论:正常大鼠心肌组织存在DR1和DR2mRNA和蛋白质的表达,心肌肥厚时其表达明显减低,两者的关系及可能机制有待于进一步研究。更多还原

【Abstract】 AIM: To observe the expression profile of DR1, DR2 mRNA and protein in rat heart during pathological cardiac hypertrophy. METHODS: Hypertrophy model was established using renal artery coarctation (AC). Hypertrophy coefficient, left ventricular pressure and collagen were determined 35 days after the operation. RT-PCR, in situ hybridization, immunofluorescence, Western blotting, and integrating image analysis system were applied to examine DR1, DR2 mRNA and protein expressions and location in rat heart. RESULTS: mRNA and protein of DR1 and DR2 were detected in normal rat heart. DR2 mRNA in vascular smooth muscle cells was higher than that in ventricular muscle and the atrium muscle cells. Compared with the normal group, DR1, DR2 mRNA and protein were remarkably decreased in AC group than those in control group. CONCLUSION: Dopamine receptor D1, D2 mRNA and protein expresse in the normal rat heart and their expression decreases in cardiac hypertrophy.更多还原