【摘要】 目的:观察体外压力培养对人脐静脉内皮细胞(HUVECs)非对称性NG-二甲基精氨酸(asymmetricNG,NG-dimethyl-L-arginine,ADMA)代谢的影响。方法:分大气压(APC,0mmHg),中压(MPC,120mmHg)和高压(HPC,180mmHg)三组,各组分别用卡托普利(Cap,10μmol/L或100μmol/L)和厄贝沙坦(Irb,10μmol/L或100μmol/L)进行干预。测定β-N-乙酰氨基己糖苷酶活性以反映12h细胞增殖。上清液用以测定ADMA的浓度(高效液相色谱法,HPLC)。结果:①中、高压组ADMA浓度显著高于大气压组(0.75±0.05vs4.69±0.37和4.48±0.39,P<0.01),两压力组间无明显差异。②大气压时Cap与Irb对ADMA浓度无明显影响,而中、高压组药物使ADMA增加幅度减轻,且呈浓度依赖性趋势。结论:压力刺激可使条件培养液中ADMA的水平增加。抑制肾素-血管紧张素系统(renin-angiotensinsystem,RAS)可以减轻ADMA增加,表明RAS激活参与压力诱导的ADMA代谢异常。更多还原

【Abstract】 AIM: To observe the effects of high hydrostatic pressure on asymmetric NG, NG-dimethyl-L-arginine (ADMA) metabolism of human vascular endothelial cells (HUVECs), and the role of renin-angiotensin system (RAS). METHODS: Cultured HUVECs of 3-6th passage were exposed to atmosphere (0 mmHg, APC), 120 mmHg (MPC), 180 mmHg (HPC). There were three groups in each pressure condition, one as control, the other two were interfered with captopril (Cap, 10 μmol/L or 100 μmol/L) or irbesartan (Irb, 10 μmol/L or 100 μmol/L) respectively. Cell proliferation was quantified by determining hexosaminidase activity at 12 h. Concentration of ADMA in conditioned medium was measured by high performance liquid chromatography (HPLC) at 12 h. RESULTS: Compared with APC group, ADMA concentration increased prominently in MPC and HPC (4.69±0.37 and 4.48±0.39 vs 0.75±0.05,P<0.01), but no difference was found between MPC and HPC group. ADMA concentration was not influenced by Cap and Irb in APC, but obviously reduced in MPC and HPC in a dose-dependent manner. CONCLUSION: ADMA is upregulated by high hydrostatic pressure and RAS is involved.更多还原