【摘要】 目的:观察复方丹参滴丸对过氧化氢(H2O2)损伤人脐静脉内皮细胞(HUVEC)的保护作用,探讨其在心脑血管疾病治疗中的机制。方法:用0.1 mmol/L H2O2制造HUVEC损伤模型;按分组把不同浓度的丹参滴丸(0.5g/L0、.25 g/L、0.1 g/L)分别于损伤前、损伤后加入,分别测定细胞活力(MTT法)观察滴丸对H2O2损伤内皮细胞活性的影响;硫代巴比妥酸法测定细胞内脂质过氧化产物丙二醛(MDA)含量;硝酸酶还原法测定细胞培养液中一氧化氮含量;免疫细胞化学法观察滴丸对H2O2损伤的内皮细胞NOS2、NOS3(一氧化氮合酶2、3)、NF-κB(核因子кB)表达的影响;同时进行形态学观察。结果:0.1 mmol/L H2O2对HUVEC有损伤作用。H2O2损伤后,滴丸能显著增加细胞活性;显著抑制H2O2诱导HUVEC MDA的产生;双向调节NO产生;影响H2O2损伤后细胞NOS3、NF-κB的表达。结论:损伤前给滴丸浓度0.5 g/L、0.25 g/L、0.1 g/L对H2O2所致内皮细胞损伤有明显拮抗作用。损伤后给药滴丸浓度0.5g/L0、.25 g/L、0.1 g/L对H2O2所致人血管内皮细胞损伤有明显拮抗作用,其作用机制与抗氧化作用及调节NOS2、NOS3、NF-κB的表达有关。更多还原

【Abstract】 AIM: To observe the protective effect of composite salviae dropping pills against human umbilical vein endothelial cells（HUVECs） injure induced by hydrogen dioxide（H₂O₂） and discuss the mechanism of its therapeutic action on cardiac and encephalic diseases.METHODS: HUVECs were injured by 0.1 mmol/L H₂O₂ and then different final concentrations of composite salviae dropping pills（0.5 g/L,0.25 g/L,0.1 g/L） were added before and after the injury.Cell viability was measured by MTT assay.TBA method was used to detect the intracellular malonaldehyde. Nitric oxide（NO） in the culture medium was detected by using nitrate reductase assay,and immunocytochemisty was used to observe the expression of NOS2,NOS3 and NF-κB.Morphologic observation was also performed.RESULTS: HUVECs were injured by 0.1 mmol/L H₂O₂.Composite salviae dropping pills increased the cell viability apparently,inhibited the production of MDA induced by H₂O₂,regulated the generation of NO bilaterally and influenced the expression of NOS3 and NF-κB.CONCLUSIONS: Composite salviae dropping pills at concentrations of 0.5 g/L,0.25 g/L and 0.1 g/L protects HUVECs from injury by H₂O₂,no matter it is be added before or affter the injury.The possible mechanism is associated with its regulating the expression of NOS2,NOS3 and NF-κB.更多还原