【摘要】 目的:探讨双歧杆菌对大鼠腹腔巨噬细胞核转录因子NF-κB和IκB调控。方法:收集大鼠腹腔巨噬细胞,实验分为正常对照组、双歧杆菌刺激组,以不同浓度的双歧杆菌106、1071、08和109cells/L刺激细胞60 min,或以108cells/L的浓度刺激细胞不同时间(15、30、601、20和180 min),分别收集细胞以提取总蛋白及核蛋白。用凝胶电泳迁移分析法(EMSA)测定NF-κB的结合活性;用Western印迹分析巨噬细胞胞浆内相应的IκBα蛋白表达。结果:双歧杆菌处理后,巨噬细胞NF-κB向核内转移,结合活性明显增加,相应的IκBα表达降低。结论:双歧杆菌可以通过激活腹腔巨噬细胞核转录因子NF-κB来发挥调作控用。更多还原

【Abstract】 AIM: To explore the regulatory effect of bifidobacteria adolescence on NF-κB in murine peritoneal macrophages(PMs).METHODS: Macrophages were collected and were divided into tow groups,normal control group and bifidobacterium stimulation groups.The cells were fixed at 60 min after stimulated with bifidobacterium at 10~6,10~7, 10~8 and 10~9 cells/L,or fixed respectively at 15,30,60,120 and 180 min after stimulated with bifidobacterium at 10~8 cells/L,then the total protein and nucleoprotein were extracted.The activities of NF-κB and IκBα were measured with the methods of MSA and Western blotting.RESULTS: NF-κB activity markedly increased and reached the peak at 0.5 h after stimulation,while IκBα markedly decreased at same time.CONCLUSION: NF-κB activity is markedly activated by bifidobacterium.NF-κB pathway participates in the regulation of peritoneal macrophage in this process.更多还原