【摘要】 目的:探讨甘氨酸对急性坏死性胰腺炎鼠血和胰腺组织中TNF-α、IL-1β、IL-6、IL-8和IL-10的影响。方法:Wistar大鼠150只,随机分为3组:假手术组(SO)、急性坏死性胰腺炎组(ANP)和急性坏死性胰腺炎+甘氨酸处理组(ANP+Gly),以牛磺胆酸钠复制急性坏死性胰腺炎大鼠模型,ANP+Gly组大鼠胰管内给予牛磺胆酸钠前10min静脉给予甘氨酸1g/kg。观察血和胰腺组织中ET、TNF-α、IL-1β、IL-6、IL-8和IL-10含量变化,同时观察各组大鼠胰腺组织的病理变化、24h病死率及平均生存时间。结果:在0h、2h、4h、8h和12h的血和胰腺组织中ET、TNF-α、IL-1β、IL-6、IL-8和IL-10水平,ANP组和ANP+Gly组均显著高于SO组(除0h组及ANP组的IL-1012h外)(P<0·01/P<0·05),且在ANP和ANP+Gly组ET、TNF-α、IL-1β、IL-6和IL-8随病程进展而升高(P<0·05),在ANP组IL-10水平随病程进展降低(P<0·05),而在ANP+Gly组IL-10水平随病程进展增高(P<0·05)。在相同时段除ET在ANP组和ANP+Gly组无显著差异外(P>0·05),除0h组外,TNF-α、IL-1β、IL-6和IL-8水平在ANP组显著高于ANP+Gly组(P<0·01),而IL-10水平(除0h和2h组无差异外)ANP+Gly组显著高于ANP组(P<0·05/0·01)。虽然ANP组和ANP+Gly组大鼠胰腺组织的病理变化无显著差异,但ANP+Gly组大鼠24h病死率显著低于ANP组(P<0·05),平均生存时间显著延长于ANP组(P<0·05)。结论:ET、TNF-α、IL-1β、IL-6、IL-8和IL-10参与了大鼠ANP的病理过程,甘氨酸可减少促炎细胞因子TNF-α、IL-1β、IL-6和IL-8的产生,上调抗炎细胞因子IL-10的负调控作用,有助于减轻ANP时炎性细胞因子瀑布样级联反应,降低大鼠24h病死率及延长平均生存时间。更多还原

【Abstract】 AIM: To evaluate the effect of glycine on the changes of TNF-α, IL-1β, IL-6, IL-8 and IL-10 in rat serum and pancreas tissue with acute necrotizing pancreatitis (ANP). METHODS: 150 Wistar rats were randomly divided into sham operation group (SO), ANP group and ANP+glycine group (ANP+Gly). The ANP model was established by retrograde injection of sodium taurocholate into cholangiopancreatic duct. The glycine (1 g/kg) was injected intravenously 10 min before sodium taurocholate injection. The changes of ET, TNF-α, IL-1β, IL-6, IL-8 and IL-10 in serum and pancreas tissue were determined and the pathological study was performed as well. RESULTS: The levels of ET, TNF-α, IL-1β, IL-6, IL-8 and IL-10 in serum and pancreas tissue (except IL-10 at 0 h and 12 h in ANP group) in ANP and ANP+Gly groups were significantly higher than those in SO (except at 0 h) (P<0.01, P<0.05). The levels of ET, TNF-α, IL-1β, IL-6 and IL-8 were significantly increased in iso-group aniso-time, but the levels of IL-10 were significantly decreased in aniso-time of ANP group and significantly increased in aniso-time of ANP+Gly group (except at 0 h) (P<0.05). Although the levels of ET had not significant difference between group ANP and ANP+Gly, the levels of TNF-α, IL-1β, IL-6 and IL-8 in group ANP were significantly higher than that in group ANP+Gly (except at 0 h) (P<0.01). The levels of IL-10 in group ANP+Gly (except at 0 h and 2 h) were significantly higher than that in group ANP (P<0.01). The pathological change in group ANP and ANP+Gly had not significant difference, but at 24 h fatality rates and average survival time in group ANP+Gly were obvious higher than that in group ANP (P<0.05). CONCLUSION: Glycine decreases the production of TNF-α, IL-1β, IL-6 and IL-8 and up-regulates the expression of IL-10, decreases the 24 h fatality rates and improves the average survival time in rats with ANP.更多还原