【摘要】 目的:研究具有诱导肿瘤细胞凋亡活性的冬凌草甲素,促进巨噬细胞对因凋亡的肿瘤细胞的吞噬作用。方法:DNA凝胶电泳检测UV照射(2·4J/cm2,4min)的人组织淋巴瘤U937细胞凋亡;Giemsa染色,Hoechst33258染色,镜下检测计数吞噬作用。结果:UV照射(2·4J/cm2,4min)诱导U937细胞发生凋亡,琼脂糖凝胶电泳可见凋亡典型的DNA梯带。2·7μmol·L-1的冬凌草甲素具有增强U937分化的巨噬细胞对UV照射诱导凋亡的U937细胞的吞噬作用,并呈时间剂量依赖性,但对非特异性荧光颗粒的吞噬效果较弱。加入抗TNFα和抗IL-1β的抗体,培养12h,吞噬增强作用明显受抑制。冬凌草甲素在人外周血来源的巨噬细胞吞噬凋亡的U937细胞过程中同样发挥增强吞噬的效果。结论:冬凌草甲素可特异地增强巨噬细胞对凋亡的U937细胞的吞噬作用,其吞噬机制是通过诱导巨噬细胞TNFα和IL-1β的释放。更多还原

【Abstract】 AIM: To study the effect of oridonin on the phagocytosis of apoptotic U937 cells by macrophage-like cells. METHODS: DNA agarose gel electrophoresis, Giemsa staining, Hoechst 33258 staining and photomicroscopical observation were used. RESULTS: UV irradiation (2.4 J/cm~2, 4 min) induced U937 cell apoptosis. Marked DNA fragmentation in agarose gel electrophoresis was observed. Oridonin augmented phagocytosis of apoptotic U937 cells by U937 cell-derived macrophages in a time- and dose-dependent manner. However, less effect on synthetic fluoresbrite micropheres was observed. The oridonin-augmented phagocytosis was attenuated by anti-human TNFα or anti-human IL-1β antiserum. In addition, the similar effect of phagocytosis was observed in oridonin-treated human monocyte-derived macrophages at 4 day maturation. CONCLUSION: Oridonin enhances phagocytosis of apoptotic U937 cells by macrophage-like cells. The releases of TNFα and IL-1β are involved in this mechanism.更多还原