【摘要】 目的:探讨糖基化终产物(AGEs))对人单核细胞源树突状细胞(MDCs)糖基化终产物受体(RAGE)表达的影响。方法:用免疫磁珠分离人外周血CD14+单核细胞,经含rhGM-CSF(100μg/L)和rhIL-4(50μg/L)的RPMI-1640培养,使其分化为MDCs,采用RT-PCR和Westernblotting法,观察糖基化-白蛋白(AGE-BSA)对MDCsRAGEmRNA和蛋白表达的影响,同时检测培养液上清中IFN-γ和IL-12的浓度。结果:AGE-BSA诱导DCsRAGEmRNA和蛋白的表达(P<0.05),高于空白对照组,并且明显促进了DCsIFN-γ和IL-12的分泌(P<0.05)。BSA干预组与空白对照组相比差异无显著(P>0.05)。结论:AGEs能够上调DCsRAGE的表达,并且促进了DCsIFN-γ和IL-12的分泌,这可能是糖尿病通过DCs促进动脉粥样硬化发生的重要机制之一。更多还原

【Abstract】 AIM: To investigate the effect of advanced glycosylation end products on the expression of receptor for advanced glycosylation end products in human monocyte-derived dendritic cells. METHODS: Monocytes were purified (over 98%) using anti-CD14+ microbeads. After 8 d culture in RPMI-1640 medium containing rhGM-CSF (100 μg/L) and rhIL-4 (50 μg/L), immature MDCs were derived, then exposed to AGE-BSA (0 or 200 mg/L) for 24 h. Expression of RAGE was semi-quantified by RT-PCR and Western blotting. At the same time, supernatants were collected. IFN-γ and IL-12 were analyzed by ELISA. RESULTS: mRNA and protein of RAGE incubated by 200 mg/L AGE-BSA was higher than that in control at 24 h. Treatment of DCs with AGE-BSA resulted in about two-fold increase in the expression of RAGE (P<0.05). The concentrations of IFN-γ and IL-12 were both significantly higher than that in control (P<0.05). CONCLUSION: AGEs up-regulates the expression of RAGE and induces the secretion of IFN-γ and IL-12 by DCs. These findings may provide insight into the effect of DCs on the processes of atherosclerosis.更多还原