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淡紫拟青霉NH-PL-03菌株的多糖及其对尖孢镰刀菌的抑制作用

Ploysaccharides of Paecilomyces lilacinus strain NH-PL-03 and its inhibitory effects on Fusarium oxysporum

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【作者】 史怀朱育菁李芳刘波

【Author】 SHI Huai1, ZHU YU-jin1, LI Fang2, LIU Bo1 (1. Biotechnology institute, Academy of Agricultural Science of Fujian Province, Fujian Fuzhou, 350003, China; 2. College of Plant Protection, Fujian Agriculture and Forest University, Fujian Fuzhou, 350002, China)

【机构】 福建省农业科学院生物技术研究所福建省农林大学植保学院福建省农业科学院生物技术研究所 福建福州350003福建福州350003350002

【摘要】 提取淡紫拟青霉NH-PL-03菌株的胞内、外多糖,分别以菌丝生长法与孢子萌发法研究其对尖孢镰刀菌的抑制效果。结果表明,淡紫拟青霉多糖对尖孢镰刀菌有明显抑制作用。在PDA平板上观察到不同程度的抑制现象,并在显微镜下观察到尖孢镰刀菌菌丝局部膨大,细胞质浓缩,细胞壁消解等现象。淡紫拟青霉多糖对尖孢镰刀菌孢子萌发有强烈抑制作用,并随多糖浓度的提高而增强。

【Abstract】 Intra-and inter-cellular polysaccharides of Paecilomyces lilacinus (PLP) were extracted and treated with Fusarium oxysporum to test its inhibitory effects on hypae growth and condium germination. The results indicated that PLP had remarkable inhibitory effects on the growth of this pathogen. It was observed that the growth of Fusarium oxysporum was inhibited in various degrees on PDA plates, partial hypae swelling, cytoplasm condensation and cell-wall collapse could be detected under microscope and the levels of condium bourgeoning block were increasing along with the increments of PLP concentrations.

【基金】 国家863计划项目(2002AA244031-2);福建省计委项目[闽计农经(2002)48号]
  • 【文献出处】 中国植保导刊 ,China Plant Protection , 编辑部邮箱 ,2006年09期
  • 【分类号】S476
  • 【被引频次】47
  • 【下载频次】306
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