【摘要】 采用MTT法检测BTV-HbC3对Hep-3B细胞的增殖抑制作用,流式细胞术检测BTV-HbC3诱导Hep-3B细胞的凋亡情况,透射电镜观察感染BTV-HbC3的Hep-3B细胞超微结构变化。结果表明BTV-HbC3对Hep-3B细胞具有抑制效应,并呈浓度和时间依赖性;BTV-HbC3作用下Hep-3B细胞呈现凋亡特征;BTV-HbC3能有效感染人肝癌细胞株Hep-3B,并在其中有限地复制,同时抑制该细胞增殖,诱导其进入凋亡。本研究证实了蓝舌病毒HbC3株对人肝癌细胞Hep-3B的杀伤及其诱导凋亡作用,结合本室已反复证实该病毒不感染人源正常细胞的事实,提示了该病毒具有抗人肝癌之潜能。更多还原

【Abstract】 When investigating the effect of BTV-HbC3 on the hepatic carcinoma 3B (Hep-3B) cells , we detected a cytopathic effect (CPE) and inhibition activity of Hep-3B cells infected with BTV-HbC3 by using MTT, the apoptosis indicator using flow cytometry (FCM) and microstructures. The results showed that BTV-HbC3 could inhibit the Hep-3B cells proliferation moderately, which depended on the concentration of BTV-HbC3 and time.The human Hepatic carcinoma 3B cells infected by BTV-HbC3 had all the characteristic of apoptosis. Our finding indicated that oncolytic way of BTV-HbC3 was selectively replicated in tumor cells and induced apoptosis. It was further shown in our laboratory that normal human cells couldn’t be infected by this kind of virus suggesting that this virus possessed the potential function of killing the human hepatic carcinoma.更多还原