【摘要】 马尾松毛虫质型多角体病毒湖南株第9个片段编码非结构蛋白NS5,利用pET-32a(+)载体带有组氨酸标记的重组蛋白进行表达,获得纯化的重组蛋白。利用凝胶电泳阻滞检测试验(EMSA)检测蛋白和核酸的结合作用,结果表明NS5蛋白能与病毒基因组dsRNA结合。更多还原

【Abstract】 The nucleotide sequence of segment 9 of Dendrolimus punctatus cypovirus(DpCPV)strain Hunan,which encodes the viral non-structural protein(NS5),was tagged with his-tag and expressed by pET32a(+).The recombinant protein was subsequently purified to homogeneity.By using the EMSA,which allows the detection of protein-RNA interactions in the test tube,the possible interactions were tested by the recombinant protein and the double-strand RNA(the genome of DpCPV).更多还原