【摘要】 目的:探索大鼠左冠状动脉前降支不同结扎处理后,对心肌形态学及心功能的影响,以建立适合移植干细胞再生修复心肌梗死研究的稳定、可靠和更合乎发病机理的动物模型。方法:雄性W istar大鼠70只,随机分为六组即:假手术组、结扎(15 m in、30 m in、45 m in、60 m in)再灌、结扎非再灌。于处理后1 d、1周、2周或4周动态观察心肌梗死变化,并于处理一月后测量动脉收缩压(ASP)、动脉舒张压(ADP)、左室收缩压(LVSP)、左室舒张末压(LV-EDP)及左室压力上升及下降最大速度(±dp/dtm ax)。结果:引起明显的心肌梗死至少需要结扎30 m in。结扎(45m in、60 m in)再灌、结扎非再灌的心肌梗死明显,并观察到梗死区域心肌已绝大部分纤维化,且梗死面积变化较恒定。同时测定不同结扎时间心功能的变化发现,结扎(45 m in、60 m in)再灌或结扎非再灌各组ASP、ADP、LVSP、±dp/dtm ax显著下降,LVEDP明显升高。并且不同结扎时间处理后,大鼠心功能的变化与心肌梗死后的梗死面积变化密切相关。结论:建立了在实验大鼠左冠状动脉前降支中上1/3处结扎45 m in以上的大鼠心肌梗死模型,不仅合乎临床心肌梗死的发病机理,而且梗死部位、梗死区域面积稳定,适合于移植细胞再生修复心肌梗死的研究。更多还原

【Abstract】 Objective To study the feasibility of establishment of myocardial infarction models induced by different occlusion time course of left anterior descending(LAD) in rats,provide a considerably reliable model for the study of repair or rebuild rat myocardial infarction by stem cells transplantation.Methods Myocardial infarction was induced by LAD occlusion in male Wistar rats.There is 60 male Wistar rats were randomly divided into six groups: acute LAD occlusion(15,30,45 or 60 min) with reperfusion or permanent LAD occlusion group.1day,1week,2weeks or 4weeks after different LAD ligation treatment,Pathological morphology,IS and mortality were be detected and analyzed.And 1month After LAD occlusion,We used multipurpose polygraph to measure heart rate(HR),left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),±dp/dtmax,artery systolic pressure(ASP),artery diastolic pressure(ADP).Results The myocardial necrosis or infarction was formed by LAD occlusion for at least 30 minutes.Almost the same infarct area had formed in rats among occlusion(45 or 60 min) with reperfusion groups,or permanent occlusion group.However,when arterial blood flow was restored(occlusion with reperfusion),the scattered bleeding and cell damage in rat myocardial infarction area occurred.After permanent LAD occlusion or occlusion reperfusion,nearly 25% rats died of ventricular fibrillation within 4 hours,and most of them died within the first 30 minutes.The heart sections from rats of one day,one week,or one month after LAD occlusion showed the typical acute myocardial infarction pathological morphology.Compared to the LAD occlusion(15 or 30 min) with reperfusion group,ASP,LVSP and±dp/dtmax of the LAD occlusion(45 or 60 min) with reperfusion or permanent LAD occlusion group were decreased,LVEDP increased.Conclusion Our findings suggest that rat acute myocardial infarction model is the most stable by LAD occlusion(at least 45 minutes) with reperfusion and this model is suitable for the research on stem cells transplantation.更多还原