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大鼠左冠状动脉不同结扎时间对心肌梗死面积及心功能的影响

Effects of Rat Myocardial Infarction Models by Different Occlusion Time Course of LAD On Infarction Size And Heart Function

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【作者】 杨建业张迎春王明江唐俊明汤敏王家宁

【Author】 YANG Jian-ye1,ZHANG Ying-chun 2,WANG Ming-jiang3,TANG Jun-ming1,3,TANG Min4,WANG Jian-ning1*(1Institute Of Clinical Medicine,2Department of Neurology,Renmin Hospital,3Department of physiology,4 Taihe Hospital,Yunyang Medicine College,Shiyan,Hubei 42000,China)

【机构】 郧阳医学院附属人民医院临床医学研究所郧阳医学院神经内科郧阳医学院生理学教研室郧阳医学院附属太和医院郧阳医学院附属人民医院临床医学研究所 郧阳医学院生理学教研室湖北十堰442000

【摘要】 目的:探索大鼠左冠状动脉前降支不同结扎处理后,对心肌形态学及心功能的影响,以建立适合移植干细胞再生修复心肌梗死研究的稳定、可靠和更合乎发病机理的动物模型。方法:雄性W istar大鼠70只,随机分为六组即:假手术组、结扎(15 m in、30 m in、45 m in、60 m in)再灌、结扎非再灌。于处理后1 d、1周、2周或4周动态观察心肌梗死变化,并于处理一月后测量动脉收缩压(ASP)、动脉舒张压(ADP)、左室收缩压(LVSP)、左室舒张末压(LV-EDP)及左室压力上升及下降最大速度(±dp/dtm ax)。结果:引起明显的心肌梗死至少需要结扎30 m in。结扎(45m in、60 m in)再灌、结扎非再灌的心肌梗死明显,并观察到梗死区域心肌已绝大部分纤维化,且梗死面积变化较恒定。同时测定不同结扎时间心功能的变化发现,结扎(45 m in、60 m in)再灌或结扎非再灌各组ASP、ADP、LVSP、±dp/dtm ax显著下降,LVEDP明显升高。并且不同结扎时间处理后,大鼠心功能的变化与心肌梗死后的梗死面积变化密切相关。结论:建立了在实验大鼠左冠状动脉前降支中上1/3处结扎45 m in以上的大鼠心肌梗死模型,不仅合乎临床心肌梗死的发病机理,而且梗死部位、梗死区域面积稳定,适合于移植细胞再生修复心肌梗死的研究。

【Abstract】 Objective To study the feasibility of establishment of myocardial infarction models induced by different occlusion time course of left anterior descending(LAD) in rats,provide a considerably reliable model for the study of repair or rebuild rat myocardial infarction by stem cells transplantation.Methods Myocardial infarction was induced by LAD occlusion in male Wistar rats.There is 60 male Wistar rats were randomly divided into six groups: acute LAD occlusion(15,30,45 or 60 min) with reperfusion or permanent LAD occlusion group.1day,1week,2weeks or 4weeks after different LAD ligation treatment,Pathological morphology,IS and mortality were be detected and analyzed.And 1month After LAD occlusion,We used multipurpose polygraph to measure heart rate(HR),left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),±dp/dtmax,artery systolic pressure(ASP),artery diastolic pressure(ADP).Results The myocardial necrosis or infarction was formed by LAD occlusion for at least 30 minutes.Almost the same infarct area had formed in rats among occlusion(45 or 60 min) with reperfusion groups,or permanent occlusion group.However,when arterial blood flow was restored(occlusion with reperfusion),the scattered bleeding and cell damage in rat myocardial infarction area occurred.After permanent LAD occlusion or occlusion reperfusion,nearly 25% rats died of ventricular fibrillation within 4 hours,and most of them died within the first 30 minutes.The heart sections from rats of one day,one week,or one month after LAD occlusion showed the typical acute myocardial infarction pathological morphology.Compared to the LAD occlusion(15 or 30 min) with reperfusion group,ASP,LVSP and±dp/dtmax of the LAD occlusion(45 or 60 min) with reperfusion or permanent LAD occlusion group were decreased,LVEDP increased.Conclusion Our findings suggest that rat acute myocardial infarction model is the most stable by LAD occlusion(at least 45 minutes) with reperfusion and this model is suitable for the research on stem cells transplantation.

【关键词】 大鼠心肌梗死心功能梗死面积
【Key words】 RatMyocardial infarctionHeart functionInfarct size
【基金】 湖北省自然基金资助项目(2005ABA079);湖北省卫生厅项目(JX2B68);湖北省教育厅项目(Q200524003)
  • 【文献出处】 郧阳医学院学报 ,Journal of Yunyang Medical College , 编辑部邮箱 ,2006年02期
  • 【分类号】R542.22
  • 【被引频次】23
  • 【下载频次】430
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