【摘要】 以蝴蝶兰R4品种叶片为外植体,研究不同pH培养基、培养温度对褐化率、多酚氧化酶(PPO)活性和总酚含量的影响以及褐化率与PPO、总酚之间的相关性。结果表明:培养基pH6.5或培养温度20℃时外植体褐化率最低。在相同温度(25℃)下,PPO活性、总酚含量高低并不与pH值大小成正比,且褐化率与PPO无显著相关性,pH5.0时,褐化率与总酚含量显著相关;在相同pH(6.0)下,温度越高,褐化率越高,PPO活性越强,总酚含量越高,且20℃下,褐化率与PPO、总酚含量分别达到极显著、显著相关,30℃下,褐化率与总酚含量显著相关。更多还原

【Abstract】 The variety ’R4’of Phalaenopsis was used for the study of pH and temperature effect on polyphenol oxidase(PPO)activity,total phenolic content,browning rate of the explants,correlation of browning rate and PPO activity with total phenolic content.The browning rate was the lowest at pH6.5 or 20℃.Under the same temperature(25℃),the PPO activity and total phenolic content didn’t have direct ratio with the bulk of pH,and there was no significant correlation between PPO activity and total phenolic content,but there was significant correlation between browning rate and total phenolic content at pH 5.0;Under the same pH(6.0),PPO activity,total phenolic content and browning rate increased while the temperature rose,and there were the best significant correlation between browning rate and PPO activity at 20℃,significant correlation between browning rate and total phenolic content at 20℃ and 30℃.更多还原