The effects of medium composition on the in vitro culture of unfertilized ovules of a Phalaenopsis hybrid were studied. The results showed that the megasporocytes of the hybrid were capable of undergoing meiosis to form bi-nucleate embryo sacs in vitro by ovule cultures on the medium of VW + BA 0.2 mg·L~ -1 + NAA 0.2 mg·L~ -1 + sucrose 2.0%+phytagel 0.3%. On the medium of VW + BA 0.2 mg·L~ -1 + NAA 0.2 mg·L~ -1 + sucrose 2.0% + phytagel 0.3% +coconut milk 10%, the megasporocytes would develop into 4-nucleat...