【摘要】 以线艺春兰‘绿云’的类原球茎为材料,研究了植物生长调节剂等因素对根状茎的增殖与分化的影响。结果表明:长期继代培养及保存宜选用1/2MS固体培养基。细胞分裂素6-BA浓度为1·0mg·L-1时根状茎的增殖效果最佳。0~3·0mg·L-1范围内IBA促进根状茎的生长较NAA效果好。1/2MS+6-BA1·0mg·L-1+IBA1·0mg·L-1培养基最利于根状茎的分化,芽分化率达到36·9%。培养基添加芋艿糊可显著促进根状茎的增殖与生长。根状茎分割以掰开方式且接种团块带有3~5条根状茎较适宜。线艺春兰叶片上的腹轮艺通过组培可以稳定遗传。更多还原

【Abstract】 The rapid multiplication system of Cymbidium goeringii ‘Lüyun’ with verge line pattern leaves by protocorm like-body was established. Effects of plant growth regulators on multiplication and differentiation of rhizomes were studied. 1/2 MS solid medium was suitable for subculture and preservation of the rhizome. The optimal medium for multiplication and growth of rhizomes was MS+6-BA 1.0 mg·L~ -1 . IBA was more effective to the growth of rhizomes than NAA with increasing of auxin concentration (0-3 mg·L~ -1 ). The optimum medium for differentiation of rhizomes was 1/2 MS supplemented with 6-BA 1.0 mg·L~ -1 and IBA 1.0 mg·L~ -1 , which regeneration rate of shoots was as high as 36.9%. Dasheen paste was better to the multiplication of rhizomes than coconut milk and banana mud added to the culture medium. Single conglomeration with 3-5 rhizomes was optimum by the way of broking off with fingers and thumb. Verge line pattern on the leaf could be stably inherited.更多还原