【摘要】 利用RT-PCR和RACE技术,从蟠桃(Amygdaluspersicavar.compressaBean)中克隆出3285bp的β-半乳糖苷酶基因cDNA全长,GenBank登录号为AY874412。该cDNA2559bp,编码853个氨基酸。将其插入到大肠杆菌表达载体pET-32a(+)中,转化BL21trxB(DE3),筛选重组菌株。经IPTG诱导表达后,SDS-PAGE呈现约115kDa特异表达条带。并通过体外酶促反应分析表达的融合蛋白的生物活性。更多还原

【Abstract】 A 3 285 bp cDNA encoding β-galactosidase was cloned from compress peach by the methods of RT-PCR and rapid amplification of cDNA ends (RACE). The accession number is AY874412, which contains 2 559 bp, encoding a 853 residue polypeptide. The cDNA was constructed into the vector pET-32a(+)designed for recombinant strain. After IPTG induction, it was observed there was a special band about 115 kDa on SDS-PAGE. The activity of the fusion protein was analyzed by in vitro enzymatic reaction.更多还原