【摘要】 以4个马铃薯品种的茎段为外植体,在含不同浓度NaCl的培养基上直接诱导耐盐愈伤组织,筛选耐盐变异体。结果表明,不同品种的耐盐性有差异,‘东农303’和‘鄂1号’愈伤组织诱导的最大耐盐浓度为0·8%,‘费乌瑞它’为0·6%,‘夏波帝’为0·4%。盐胁迫抑制愈伤组织芽和根的分化,耐盐愈伤组织继代不宜超过8~10次。盆栽和离体鉴定表明,筛选出的耐盐变异体保持了耐盐的特性。更多还原

【Abstract】 The stem explants of four potato cultivars were used to induce calli and to screen salt-tolerant mutants in the medium containing different levels of NaCl. The results indicated that different cultivars showed different tolerance levels. To calli induction, the highest salt tolerant level is 0.8% for cv. Dongnong 303 and E 1, 0.6% for cv. Favorite and 0.4% for cv. Shepody. Salt inhibited shoot and root differentiation. The salt-tolerant calli should be subcultured for not more than 8-10 times. Salt tolerant tests in vivo and in vitro revealed that the mutants possessed salt tolerance.更多还原