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薰衣草叶片高频再生体系的建立

Establishment of High-frequency Regeneration System from Leaf Explants of Lavandula angustifolia’Munstead’

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【Author】 Xu Yaozu~(1,2),Wei Yanyu~1,Wang Xiaojun~1,Zhao Min’an~1,and Zhao Haiqing~1(()~1Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China;()~2Graduate School of Chinese Academy of Sciences,Beijing 100830,China;()~3Institute of Nuclear-agricultural Sciences,Zhejiang University,Hangzhou 310029,China)

【机构】中国科学院新疆理化技术研究所；中国科学院新疆理化技术研究所乌鲁木齐830011；中国科学院研究生院；北京100830；浙江大学原子核农业科学研究所；杭州310029；乌鲁木齐830011；

【摘要】以薰衣草叶片为外植体进行了离体再生研究。结果表明:叶片愈伤组织诱导最佳的培养基是MS+2,4-D 0.1 mg/L+6-BA 0.5 mg/L,诱导率高达100%;液体悬浮—固体培养芽分化率达92.5%,芽数/愈伤组织达6.6;正交试验筛选出芽增殖最佳培养基为MS+NAA 2.0 mg/L+6-BA 0.5 mg/L+IAA 1.0mg/L,其增殖系数高达8.7;在芽增殖培养基上可直接生根,生根率达100%。更多还原

【Abstract】 The regeneration system of Lavandula angustifolia’Munstead’in vitro was studied with leaves as explants,and the results showed the optimal medium for callus induction was MS+24-D 0.1 mg/L+6-BA 0.5 mg/L with the induction frequency of 100%.Liquid suspension-solid culture can achieve(adventitious) bud differentiation rate of 92.5% and 6.6 bud per callus.Best bud multiplying medium which was MS medium supplemented with NAA 2.0 mg/L and 6-BA 0.5 mg/L and IAA 1.0 mg/L was sifted through orthogonal experiment with multiplying coefficient of 8.7.The rooting of rootless bud could be accomplished directly in the bud multiplying medium with rooting frequency of 100%.更多还原

【关键词】薰衣草；液体悬浮—固体培养；再生植株；
【Key words】 Lavandula angustifolia； Liquid suspension-solid culture； Regenerated plantlet；

【基金】中国科学院西部行动高新技术资助项目(KGCX2-SW-506)

【文献出处】园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2006年01期

【分类号】S682.3;S573.9
【被引频次】22
【下载频次】443

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