【摘要】 采集中国吉林长白山不同海拔的4种赤杨根须样本,利用巢式PCR-RFLP方法检测丛枝菌根真菌(AMF)对样品的侵染情况,PCR结果经限制性内切酶分析.结果表明,赤杨根内AMF存在丰富的基因多样性.AMF的侵染有从宿主混乱性向宿主专一性发展的趋势.东北赤杨AMF的宿主专一性水平最强,球囊霉属已成为东北赤杨的优势侵染类群;其余3种赤杨的AMF则出现宿主混乱现象.宿主因素比海拔因素对AMF侵染有更重要的影响.更多还原

【Abstract】 In this paper,the colonization of arbuscular mycorrhizal fungi(AMF)on the root samples of 4 Alnus species in Changbai Mountains was investigated by using nested PCR-RFLP technique,and the PCR results were tested by restriction endonuclease analysis method.The results revealed that the uncultured AMF had a high genetic diversity,and the colonization had a trend from promiscuity to specialization.The AMF from Alnus mandshuica showed the greatest specialization to the host,and Glomus was the dominant colonizer of A.mandshuica.The AMF from A.sibirica var.hirsuta,A.sibirica,and A.tinctoria showed promiscuity,and host had more significant effects on the colonization of AMF than altitude.更多还原