【摘要】 目的构建水痘-带状疱疹病毒(VZV)糖蛋白E的真核表达载体。方法用PCR方法扩增VZV糖蛋白E基因,并将其克隆到真核表达载体pcDNA3.1并用双酶切和测序方法鉴定。结果扩增的目的基因包括了全长的糖蛋白E基因,长度约1.9 kb。并且成功构建了糖蛋白E基因重组表达载体。结论构建的重组表达载体基因序列正确,为进一步研究此蛋白的免疫原性打下了基础。更多还原

【Abstract】 Objective To construct the eukaryotic expression vector of varicella-zoster virus(VZV)glycoprotein E.Methods VZV glycoprotein E genes were amplified by PCR and cloned into the eukaryotic expression vector pcDNA3.1.The constructed vector was identified by double enzyme-digestive method and sequencing.Results The amplified gene,which was about 1.9 kb,included entire gene of VZV glycoprotein E.The recombinant vector was identified as pcDNA-VgE.Conclusion The constructed vector containing gene of VZV glycoprotein E can express glycoprotein E,which lays the foundation for studying immunogenicity of glycoprotein E.更多还原