【摘要】 报道了一种用于测定有机磷和氨基甲酸酯类农药的安培型生物传感器。将乙酰胆碱酯酶(AChE)固定在再生丝素粉末上,再将其与石墨粉、非导电的环氧树脂和电催化剂7,7,8,8-四氰基醌二甲烷(TCNQ)充分混合在一起,制备成生物传感器。对生物传感器的制备条件进行了优化,具有良好的重现性和回收率,当氧化乐果及克百威的质量浓度分别在5.0×10-5~5.0×10-3mg/L和5.0×10-5~1.0 mg/L范围内时,抑制率与其浓度的对数呈线性关系,检测限可分别达1.9×10-5和4.7×10-5mg/L。更多还原

【Abstract】 An amperometric biosensor for the determination of organophosphorus and carbamate pesticides is described.Acetylcholinesterase（AChE） immobilized on regenerated silk fibroin powder was thoroughly mixed with graphite powder,non-conducting epoxy resin,and electronic mediator 7,7,8,8-tetracyanoquinodimethane（TCNQ） to prepare the biosensor.The preparation conditions of the biosensor were optimized.The proposed method exhibited a good reproducibility and recovery ratio.The inhibition versus the logarithm of concentration was linear to omethoate and carbofuran over the concentration ranges of 5.0×10^-5 g/L to 5.0×10^-3 mg/L and 5.0×10^-5 g/L to 1.0 mg/L with the detection limits of 1.9×10^-5 mg/L and 4.7×10^-5 mg/L,respectively.更多还原