【摘要】 研究了民间草药甜草O ldenlandia cantonensisHow的化学成分。从乙醇提取物的乙酸乙酯洗脱部分得到新的五环三萜urs-12-en-29-αoic ac id-3β-ol。该化合物结构通过NMR、IR、MS等波谱数据综合解析确定。研究了urs-12-en-29-αoic ac id-3β-ol对重组人DNA拓扑异构酶Ⅰ、肝癌细胞BEL-7402和胃腺癌细胞MCG-803的抑制作用,效果显著,IC50值分别为12.0×10-6、6.5×10-6、8.0×10-6g/mL。由V(石油醚)∶V(乙酸乙酯)=20∶1洗脱得到的低极性成分进行了气相色谱-质谱分析,共鉴定60个化合物,其中长链脂肪族化合物,如烷烃、饱和及不饱和羧酸(酯)含量较丰富,占36.16%,萜类和甾体类化合物分别占6.42%和9.28%。更多还原

【Abstract】 The chemical composition of Oldenlandia cantonensis How,a herbal medicine is（reported）.A new pentacyclic triterpenoid,urs-12-en-29α-oic acid-3β-ol was obtained from the EtOAc-eluted fraction of the（ethanol） extract of Oldenlandia cantonensis How.The structure was elucidated by spectroscopy methods,（including） NMR（1D and 2D）,IR,and MS.Urs-12-en-29α-oic acid-3β-ol exhibited potent inhibiting effect（against） the human DNA topoisomerase Ⅰ（hTopo Ⅰ）,the cancer cell lines BEL-7402,and MCG-803, with the IC₅₀ values 12.0×10^-6,6.5×10^-6 and 8.0×10^-6 g/mL,respectively.The low（polarity） fatty oil,the（eluted） fraction of V（petroleum ether）∶V（EtOAc）=20∶1 was also quantitatively analyzed by gas chromato-（graphy） mass spectrometry（GC-MS）.As a result,60 compounds were detected.The fatty oil mainly composed of long chain aliphatics,terpene and steroid compounds,with percentages of 36.16%,6.42% and 9.28%,respectively.更多还原