【摘要】 用高效液相色谱法,以乙腈-水为流动相,梯度洗脱程序和蒸发光散射检测器对大豆低聚糖进行分析,在20 m in内将大豆低聚糖中的主要成分蔗糖、棉籽糖和水苏糖与其它组分完全分离。用外标法对3种主要成分进行定量,线性关系良好。回收率为96.5%~99.1%,相对标准偏差为0.18%~4.67%。该法操作简便,分析速度快,可作为常规检测方法应用。更多还原

【Abstract】 Soybean oligosaccharides were analyzed via high performance liquid chromatography with gradient elution of acetonitril-water and an evaporative light-scattering detector.The three important components,(sucrose),raffinose,stachyose of soybean oilgosaccharides were separated completely from other components within twenty minutes.The quantitative analysis results of the three components were achieved with the(external) standard method,and the linear relations were good.The recovery of this method is 96.5%～99.1%,the relative standard deviations are in a range of 0.18%～4.67%.Operation of the method is simple and fast,applicable to the routine analysis of industrial products.更多还原