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用紫外-可见吸收光谱研究微过氧化物酶-11与Yb³⁺相互作用机理

Interaction Mechanism between Microperoxidase-11 and Yb³⁺ via UV-Vis Absorption Spectroscopy

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【Author】 LI Mei-Lin~(a),CHEN Ting-Ting~(b),HUANG Xiao-Hua~(a),FENG Yu-Ying~(a),XING Wei~(c),LU Tian-Hong~(a,c*)(~aEnvironment Friendship Laboratory,College of Chemistry and Environment Science,Nanjing Normal University,Nanjing 210097;~bCollege of Chemistry and Chemical Engineering,Nantong University,Nantong;~cChangchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022)

【机构】南京师范大学化学与环境科学学院；南通大学化学化工学院；中国科学院长春应用化学研究所；南京师范大学化学与环境科学学院南京210097；南通；南京210097；长春130022；中国科学院长春应用化学研究所长春130022；

【摘要】用紫外-可见(UV-V is)吸收光谱研究了微过氧化物酶-11(MP-11)与Yb3+相互作用机理。结果表明,Yb3+优先与MP-11分子的血红素中2个丙酸基团的羧基氧发生键合作用,而且1个Yb3+与1个丙酸基上的羧基氧键合。这种相互作用使血红素卟啉环的非平面性增加,π-π*跃迁所需能量减少,但跃迁几率降低。由于肽链上的羰基基团被包埋在肽链的疏水基团中,Yb3+基本上不能与MP-11肽链中的羰基氧发生键合作用。更多还原

【Abstract】 The interaction mechanism between microperoxidase-11 and Yb³⁺ was studied by means of UV-Vis absorption spectroscopic technique.It was found that Yb³⁺ could bond with the carbonyl oxygen of the（metacetonic） acid group in the heme group of the MP-11 molecule.One Yb³⁺ ion links with one carbonyl oxygen of the metacetonic acid group.This kind of interaction results in the increase in the non-planarity of the porphyrin group in the heme group and the decrease in the energy for the π-π^* transition.However,the（probability） of the π-π^* transition is decreased.Yb³⁺ could not link with the carbonyl oxygen of the amido group in the peptide of the MP-11 molecule because the carbonyl oxygen of the amido group in the peptide is embedded in the hydrophobic environment in the peptide.更多还原