【摘要】 选择了内源基因大豆植物凝集素(lectin)、玉米转化酶(invertase)和外源基因花椰菜花叶病毒35S(CaMV35S)启动子的分子信标探针,确定了探针浓度和镁离子浓度等反应条件,分别对转基因大豆和转基因玉米系列标准品进行内源基因和外源基因的荧光PCR扩增,在PCR反应过程中分别以两种荧光通道信号分别追踪同一样品DNA内源基因和外源基因的扩增动力学变化,并依此绘制了循环阈值与转基因食品百分比含量之间的标准曲线,建立了转基因大豆和转基因玉米的分子信标探针-荧光定量PCR检测方法,该方法具有特异性强、敏感性高的特点,实现了对转基因食品的定量分析.图7表1参11更多还原

【Abstract】 Primers and molecular beacon probes of endogenous gene lectin, invertase, and exogenous gene CaMV 35S promoter were designed. The reaction conditions, including probe concentration and MgCl_ 2 concentration were optimized. Genetically modified soybean and genetically modified maize reference materials were detected by fluorescence PCR amplification of endogenous gene and exogenous gene. The standard curves of ΔC_ t between endogenous gene and exogenous gene vs. the transgenic-content in reference materials were generated and a linear regression equation could be obtained. Molecular beacon-fluorescence quantitative PCR method was established to detect genetically modified soybean and genetically modified maize. The method was used to quantitatively detect and identify transgenic food. Fig 7, Tab 1, Ref 11更多还原