【摘要】 采用Affymetrix的基因芯片(RAE230A)技术,研究了SO2长期动态吸入(14mg/m3,1h/d,共30d)后大鼠肺组织基因表达谱的改变.结果表明,与对照组(CK)相比,SO2长期吸入后大鼠肺组织表达上调的基因有173个,其中包括79个已知基因和94个新基因,而表达下调的基因有85个,其中包括46个已知基因和39个新基因.表明:(1)长期吸入SO2的大鼠肺基因表达谱与其CK相比,表达有差异的基因涉及到脂肪酸代谢、免疫、炎症、氧化应激、原癌基因、肿瘤抑制基因和细胞外基质等,表明低剂量长期吸入SO2在体内的机理非常复杂;(2)SO2高剂量短期吸入与低剂量长期吸入后,大鼠肺组织基因表达谱的改变很不一致,表明二者在体内的作用机理不同;(3)SO2可引起多种基因的表达发生改变,这意味着多种基因的表达是易变的、不稳定的,在肺组织中可能存在着SO2诱发的表达不稳定型基因组.表1参19更多还原

【Abstract】 Affymetrix GeneChip (RAE230A) analysis was conducted to study the gene expression profiles in the lungs of Wistar rats exposed to SO_ 2 for long time (14 mg/m 3, 1 h/day, for 30 days). It was found that 173 genes, including 79 known genes and 94 novel genes, were up-regulated, and 85 genes, with 46 known and 39 novel ones, were down-regulated in the lungs of rats exposed to SO_ 2 compared with control group. It could be concluded that (1) the discriminating genes in lungs of rats exposed to SO_ 2 for long time included those involved in fatty acid metabolism, immune, inflammatory, oxidative stress, oncogene, tumor suppresser and extracellular matrix. The mechanism of low-dose and long-term exposure to SO_ 2 was very complex; (2) SO_ 2 exerted its effects by different mechanisms in vivo at high-dose and short-term inhalation and at low-dose and long-term inhalation; and (3) expression of many genes in rat lungs was changed by SO_ 2, which implied that the gene expression was changeful and unstable, and there was a genome that its expression might be changed by SO_ 2. Tab 1, Ref 19更多还原