【摘要】 模拟5km急性低氧2h、4h、8h和24h,观察大鼠海马白介素6(IL6)和低氧诱导因子1(HIF1α)表达的时序变化.分别运用免疫组化和RTPCR分析海马CA1、CA2和CA3区IL6、HIF1α和海马HIF1αmRNA的表达水平.与对照组相比,5km急性低氧8h和24h,海马CA1区IL6水平升高14.24%(P<0.01)和7.76%(P<0.05);低氧8h,海马CA2和CA3区IL6水平上升3.1%(P<0.05)和5.1%(P<0.05).5km低氧4h、8h和24h,海马CA1区HIF1α水平分别升高3.67%(P<0.05)、5.5%(P<0.05)和6.27%(P<0.01),同时发现HIF1αmRNA在海马的表达上调.上述结果表明,5km急性低氧促进海马IL6和HIF1α的表达增强,推测HIF1α可能作为调控因子参与低氧下海马IL6的诱导表达.图5表1参21更多还原

【Abstract】 Interleukin-6 (IL-6) and hypoxia-inducible factor 1α(HIF-1α) expressions in hippocamus of rats were studied, exposing to 5 km acute hypoxia for 2 h , 4 h, 8 h and 24 h. The expressions of IL-6 and HIF-1α in hippocampus were analyzed by immunocytochemistry, while that of HIF-1α mRNA by RT-PCR. 5 km acute hypoxia for 8 h and 24 h increased the expression of IL-6 in hippocampus CA1 by 14.24% (P<0.01) and 7.76% (P<0.05), respectively. 5 km acute hypoxia for 8 h increased the expressions of IL-6 in hippocampus CA2 and CA3 by 3.1% (P<0.05) and 5.1% (P<0.05), respectively. 5 km acute hypoxia for 4 h, 8 h and 24 h enhanced the expressions of HIF-1α in hippocampus CA1 by 3.67% (P<0.01), 5.5% (P<0.05) and 6.27% (P<0.01), and increased HIF-1α mRNA expression in hippocampus. The results showed that acute hypoxia induced HIF-1α and IL-6 expressions in hippocampus. It indicated that HIF-1α might regulate IL-6 expression under acute hypoxia. Fig 5, Tab 1, Ref 21更多还原