【摘要】 目的建立安忧静颗粒中芍药苷的含量测定方法。方法采用反相高效液相色谱法,色谱柱为Hypersil-ODSC18分析柱(125mm×4mm,5μm),流动相为乙腈-0.1%磷酸(15∶85),检测波长为230nm,流速为1mL/min。结果方法的精密度和稳定性良好,芍药苷进样量在0.210～3.150μg范围内与峰面积线性关系良好,r=0.9999,平均回收率为98.08%,RSD为1.32%。结论反相高效液相色谱法简便、专属性强、重现性好,可有效控制安忧静颗粒的质量。更多还原

【Abstract】 Objective To develop a method for determination of paeoniflorin in Anyoujing granules which consists of Radix paeonike alba,Radix acanthopanacis senticost and eight other herbs.Methods RP-HPLC with Hypersil-ODS C18(125 mm×4 mm,5 μm)column was used.The mobile phase of paeoniflorin was a mixture of acetonitrile-0.1 mol/L phosphoric acid(15 ∶85),with the flow rate of 1.0 mL/min and detection wavelength at 230 nm.Results The linear range was from 0.210-3.150 μg(r=0.999 9)for paeoniflorin.The average recovery rate of paeoniflorin was 98.08%(RSD=1.32%).Conclusion The method is convenient,selective and reproducible for determination of paeoniflorin in Anyoujing granules.更多还原