【摘要】 目的研究联合使用血管内皮生长因子(VEGF)和地塞米松对胎鼠肺泡Ⅱ型细胞发育和肺泡表面活性物质结合蛋白B(SP-B)表达的影响,为防治早产儿呼吸窘迫综合征寻找新途径。方法取孕20 d的W istar大鼠剖腹取胎鼠,原代培养肺泡Ⅱ型细胞,将所得肺泡Ⅱ型细胞分成4组,分别加入VEGF、地塞米松、VEGF+地塞米松、空白培养液(即对照组),孵育后测定各组胎鼠肺泡Ⅱ型细胞VEGF及其受体F lt-1、F lk-1和SP-B。结果VEGF组、地塞米松组与VEGF+地塞米松组SP-B均阳性表达,对照组SP-B阴性表达;VEGF组与VEGF+地塞米松组VEGF、F lt-1、F lk-1阳性表达,地塞米松组VEGF、F lt-1、F lk-1阴性表达。结论VEGF能促进肺泡发育和肺表面活性物质SP-B的合成与分泌,拮抗地塞米松下调肺泡Ⅱ型细胞受体表达的作用。更多还原

【Abstract】 Objective To study the effects of vascular endothelial growth factor(VEGF) combined with dexamethasone on expression of pulmonary surfactant protein B so as to explore a new route to prevent and cure respiratory distress syndrome(RDS) in premature infants. Methods Rat embryos were got from Wistar rats at 20 days gestation period.Primary culture of AEC II was done.The experiment was divided into VEGF group,dexamethasone group,VEGF plus Dexamethasone group and control group to determine the amount of VEGF with its receptors Flt-1 and Flk-1 and expression of pulmonary surfactant protein B by immunology histochemistry. Results SP-B showed prositive expression in VEGF group,dexamethasone group and VEGF plus dexamethasone group.Control group showed negative expression of SP-B.VEGF group and VEGF plus dexamethasone group showed positive expression of VEGF,Flt-1 and Flk-1.Dexamethasone group showed negative expression of VEGF,Flt-1 and Flk-1. Conclusion VEGF can promote the synthesis and secretion of pulmonary surfactant protein B and antagonise the under-regulation of AEC II receptors expression by dexamethasone.VEGF and dexamethasone can improve the function of pulmonary epithelial cells through promoting the expression of pulmonary surfactant protein B.更多还原