【摘要】 目的观察TGF-β1在肾小管间质纤维化过程中的表达以及百令胶囊对TGF-β1表达的影响,探讨百令胶囊肾脏保护作用的可能机制。方法雄性3月龄SD大鼠90只,随机分为:对照组(30只),模型组(30只),预防组(30只)。以2%腺嘌呤淀粉混悬液150mg·kg-1·d-1灌胃,连续17周,制作大鼠肾小管间质纤维化模型;预防组加以1.5g·kg-1·d-1百令胶囊溶于生理盐水灌胃以预防肾小管间质纤维化;对照组以等体积的2%淀粉溶液和生理盐水灌胃。分别于实验第7周、12周、17周时随机处死10只大鼠,进行血肌酐(Cr)、尿素氮(BUN)、24h尿蛋白定量和尿NAG酶检测,光镜下观察肾组织的病理变化,计算肾小管间质纤维化指数;免疫组织化学法检测肾脏TGF-β1的表达情况。结果①功能学变化:与对照组比较,各时相点模型组、预防组大鼠尿蛋白、尿NAG酶、血Cr及血BUN均升高(P<0.01),但预防组低于模型组(P<0.01);模型组、预防组自身对照各指标均较前1时相点升高(P<0.01)。②病理学变化:7周时模型组、预防组即有肾小管间质损害;模型组、预防组自身对照肾小管间质损害均较前1时相点加重(P<0.01),但7周、12周时预防组肾小管间质损害均较模型组为轻(P<0.01),17周时两组间无显著差异(P=0.670)。③免疫组化:各时相点模型组、预防组大鼠肾脏TGF-β1的表达均高于对照组(P<0.01),且预防组均低于模型组(P<0.01),模型组、预防组自身对照均较前1时相点升高(P<0.01),17周时表达量最高。结论肾小管间质纤维化过程中肾脏TGF-β1的表达逐渐上升。百令胶囊可能通过抑制TGF-β1,在肾小管间质纤维化早期应用时起到保护作用。更多还原

【Abstract】 Objective To observe the expression of transforming growth factor-beta1 in the rats with tubulointerstitial fibrosis to explore the possible mechanism of renal-protecting of Bailing capsules. Methods 90 male Sprague-Dawley(SD) rats, aged three months, were randomly divided into three groups: control group(n=30), model group(n=30), treatment group(n=30). The renal tubulointerstitial fibrosis model was established by gavage with 150mg·kg-1·d-1 adenine solved by a solution of 2 percent Starch and the same volume Starch was gave to the rats from control group. At week 7, 12, 17, ten rats of every group were killed, the urinary protein, N-acetyl-β-D-glucosaminidase(NAG) in the urine and renal profile including BUN and serum Creatinine was examined. The histological changes of kidney were observed by light microscopy, and the expression of TGF-β1 was examined by immunohistochemistry staining. Results At week 7, 12 and 17, the value of urinary protein, urinary NAG, serum BUN and serum Cr of rats from model and treatment group were higher than those of control one(P<0.01), but those of treatment group was lower than those of model group(P<0.01). The value of urinary protein, urinary NAG, serum BUN and serum Cr of rats from model and treatment group were increase(P<0.01), the highest in the 17th week. Renal interstitium injury was seen in model group and treatment group rats at week 7, and became aggravating after week 7. The degree of renal interstitium fibrosis in rats from treatment group was lighter than that of model group at week 7 and 12 (P<0.01), but there was not a significantly difference at week 17 (P=0.670); The expression of TGF-β1 in rats from model and treatment group were higher than those of control group (P<0.01), and treatment group was lower than model group(P<0.01). The expression of TGF-β1 in rats from model and treatment group were increase, and the highest in the 17th week. Conclusions These results suggest that the increase in TGF-β1 at tubulointerstitial fibrosis(TIF). Bailing capsules suppressed fibrogenic events accompanied by a decreased in TGF-β1 expression at the early stage of TIF.更多还原