【摘要】 目的研究人乳头状瘤病毒16型(HPV16)E6/E7蛋白与结核杆菌热休克蛋白70(HSP70)表达重组体DNA免疫对小鼠活化脾细胞分泌活性的影响。方法采用肌肉注射DNA的方法免疫小鼠,分离脾细胞,用ELISA检测细胞因子的表达水平及抗体的水平,用RT-PCR的方法检测细胞中细胞因子mRNA的水平。结果以HPV16E6/E7为基础的DNA免疫小鼠后,检测到的细胞因子IL-2,IFNγ的含量明显升高;与结核杆菌HSP70重组后,IL-2,IFNγ的含量较重组前明显升高。与结核杆菌HSP70重组后,IL-10及抗体水平没有明显变化。结论以HPV16E6/E7为基础的DNA免疫能增强小鼠的细胞免疫反应。与结核杆菌HSP70重组后能提高细胞免疫的效果,但对体液免疫几乎没有影响。更多还原

【Abstract】 Objective To explore the secretive activities of mice spleen cells induced by DNA recombinant of HSP70 of mycobacterium tuberculosis and HPV16 E6/E7.Methods DNA recombinants were vaccinated to mice through muscle injection.Spleen cells were isolated.Cytokines and antibodies were detected by ELISA method.mRNAs of cytokines were detected by RT-PCR method.Results Secretions of cytokines IL-2,IFNγ were increased induced by DNA vaccinations based on HPV16 E6/E7.The quantity of increase by DNA recombinants of HSP70 of mycobacterium tuberculosis and HPV16 E6/E7 is much higher than that induced by DNA vaccinations without recombinated with HSP70.However,secretions of IL-10 and antibodies remained.Conclusion Cellular immunity in mice could be enhanced induced by DNA vaccines based on HPV16 E6/E7.It could be much stronger induced by DNA recombinants of HSP70 of mycobacterium tuberculosis and HPV16 E6/E7.However, humoral immunity remained.更多还原