【摘要】 目的比较两种不同去白细胞过滤的速率对机采浓缩血小板(plateletapheresisconcentrates,PCs)质量的影响。方法应用MCS+机型、C5版本软件程序和采集管路中连接LRF系列血小板专用去白细胞滤器。PCs在采集过程中以5~8ml/min连续性低速过滤(L-LDP)或在采集结束后PCs以25ml/min一次快速通过滤器(F-LDP)。PCs产品质量预期分别为:血小板产量≥2.5×1011细胞数/袋和残余白细胞(WBC)数≤5.0×105/袋。结果L-LDP和F-LDP方法获得的PCs产品的血小板产量分别为(2.70±0.29)×1011/袋和(2.56±0.38)×1011/袋(t=1.25),残余WBC数为(7.73±0.01)×104和(2.02±3.05)×105(logt=1.48),采集成功率为78.6%和70.6%,以及采集效率为(57.1±6.8)%和(55.5±8.7)%(t=0.64)。L-LDP法获得PCs产品同时满足血小板产量和残余WBC数的双预期值的总体率占78.6%(22/28),而L-LDP仅为64.7%(11/17)。结论两种去白细胞滤除方法都可以获得满意的效果。由于L-LDP的个性观察指标均好于F-LDP,提示慢速过滤获得的PCs产品质量更加稳定。更多还原

【Abstract】 Objective To compare the quality of platelet concentrates (PCs) between two different flow speeds of leukodepletion through filtration processes.Methods MCS+version C5 and disposables were used,each of which linked with a leuko-depleted filter of LRF series products,The PCs was gone respectively through a continuous slow filtration process at flow rate about 5 to 8 ml per minute in collection process (L-LDP) and through a fast filtration process at flow rate about 25 ml per minute after collection (F-LDP). The anticipation for PCs yield was ≥2.5×10 11 cells/bag and for residual white blood cells (WBC) was≤5.0×105 cells/bag.Results The PCs products obtained by L-LDP and F-LDP correspondingly showed the mean platelet yield (2.70±0.29)×10 11 cells/bag and (2.56±0.38)×10 11 cells/bag (t=1.25) the residual WBC (7.73±0.01)×104 cells/bag and (2.02±3.05)×105 cells/bag (log t=1.48), the success collection rate 78.6% and 70.6%, as well as the collection efficiency 57.1±6.8% and 55.5±8.7% (t=0.64) against precount. As both anticipated criteria of platelet yield and residual WBC level to be met synchronously, the PCs for L-LDP accounted for 78.6% (22/28) and the one for F-LDP hand only access to 64.7% (11/17).Conclusion The satisfactory results for PCs were obtained through both different leukodepleted filtration processes. As all individualiged indexes observed of L-LDP excelled that of F-LDP, the PCs filtrated at low speed had a better quality than the one at fast speed.更多还原