【摘要】 目的分析牡丹皮中的化学成分。方法应用优化的高效液相色谱-质谱联用方法对牡丹皮供试品进行分析。结果较好地分离了单萜苷、鞣质和酚酮类等38种化学成分。在负离子检测模式下,由电喷雾质谱得到各成分的准分子离子峰,再根据串联飞行时间质谱获得进一步的结构信息,推测出其中38种主要成分的可能结构。并对牡丹皮中主要化学成分的质谱裂解规律进行了分析。结论本方法能快速检测牡丹皮的化学成分。更多还原

【Abstract】 Aim To analyze the chemical constituents of Cortex Moutan by liquid chromatography coupled with electrospray ionization mass spectrometry.Methods An on-line optimized HPLC-DAD/MS~2 technique was employed.Results In the negative ion detection mode,38 components such as monoterpene glucosides,galloylglucoses and acetophenones were isolated.Among them,over thirty compounds were identified,including paeonol,paeonilflorin, oxypaeoniflorin,benzoylpaeoniflorin,benzoyloxypaeoniflorin,galloylpaeoniflorin,galloyloxypaeoniflorin,mundanpioside A,C,D,E,H,etc.by the high performance liquid chromatography with diode-array detection in parallel with electrospray ionization and quadrupole-time of flight tandem mass spectrometry(HPLC-DAD/ESI-MS~2).Conclusion This method can be used to rapidly determine the constituents of Cortex Moutan.更多还原