【摘要】 [目的]利用重组汉坦病毒核蛋白及其单克隆抗体的酶标记物建立IgM捕获ELISA诊断试剂。[方法]制备、纯化重组核蛋白抗原和抗核蛋白单抗并进行辣根过氧化物酶标记,在抗人μ链包被板中结合四甲基联苯胺-过氧化氢(TMB/H2O2)酶底物系统,从血清中检测肾综合征出血热早期特异性IgM抗体,并与免疫荧光抗体法(IFA)进行比较。[结论]检测42份可疑HFRS病人血清,ELISA法和IFA法均阳性的20份,均阴性的19份;ELISA法阴性、IFA法阳性的1份,ELISA法阳性I、FA法阴性的2份,2种方法的差异无统计学意义(配对χ2=0.08,P>0.05)。[结论]用重组汉坦病毒核蛋白及其单克隆抗体的酶标记物建立IgM捕获ELISA诊断试剂可用于HFRS的早期IgM抗体检测。更多还原

【Abstract】 [Objective]Establishing IgM capturing ELISA assay with nucleocapside protein of Hantaan virus and its McAb labeled with horseradish peroxidase.[Methods]Preparation and purification of recombining nucleocapside protein（rNP） antigen（Ag） and its McAb labeled with horseradish peroxidase（HRP）,with enzyme substrate system（TMBH₂O₂） play as a tracer we can detect anti-HFRS virus IgM from sera by means of polystyrene plate coated with the sheep anti-human μ chain.Result compared to that detected by immunofluorescence antibody assay（IFA）.[Results]Detected 42 positive sera and 42 negative sera samples identified by IFA with this IgM capturing ELISA reagent,it shows that the result from two method has no significant difference.[Conclusion]The IgM capturing ELISA reagent involved recombined nucleocapside protein antigen and its McAb labeled with HRP can be used in detecting sera specific IgM antibody to diagnoses of hemorrhagic fever with renal syndrome in early infection stage.更多还原