Investigation on Acute Biochemical Effects of Ce（NO₃）₃ on Liver and Kidney Tissues by MAS ¹ H NMR Spectroscopic-Based Metabonomic Approach

【Abstract】 High resolution magic angle spinning （MAS）-1H nuclear magnetic resonance （NMR） spectroscopic-based metabonomic approach was applied to the investigation on the acute biochemical effects of Ce（NO₃）₃. Male Wistar rats were administrated with various doses of Ce（NO₃）₃ （2, 10, and 50 mg·kg -1 body weight）, and MAS 1H NMR spectra of intact liver and kidney tissues were analyzed using principal component analysis to extract toxicity information. The biochemical effects of Ce（NO₃）₃ were characterized by the increase of triglycerides and lactate and the decrease of glycogen in rat liver tissue, together with an elevation of the triglyceride level and a depletion of glycerophosphocholine and betaine in kidney tissues. The target lesions of Ce（NO₃）₃ on liver and kidney were found by MAS NMR-based metabonomic method. This study demonstrates that the combination of MAS 1H NMR and pattern recognition analysis can be an effective method for studies of biochemical effects of rare earths.更多还原