【摘要】 目的棕榈氯霉素卵磷脂O/W型微乳制剂的制备与含量检测。方法用十六酸异丙酯溶解棕榈氯霉素作油相,卵磷脂和吐温80混合作为表面活性剂,乙醇为助表面活性剂,制备O/W型微乳制剂;用高效液相色谱法进行棕榈氯霉素含量的测定。色谱条件:C18柱(250 mm×4.60 mm,5μm),流动相为甲醇液,柱温25℃,流量为1.0 ml.min-1,检测波长为271 nm,进样体积20μl。结果取得了较好的棕榈氯霉素卵磷脂O/W型微乳制剂及较好的含量检测方法。讨论棕榈氯霉素可以制成卵磷脂O/W型微乳制剂,用高效液相色谱法进行含量测定。更多还原

【Abstract】 AIM To prepare O/W microemulsions of chloramphenicol palmitate in phosphatidylcholine.METHODS Using dissolved chloramphenicol palmitate in isopropyl palmitates as oil phase,lecithin and tween-80 as surfactant and alcohol as cosurfactant to prepare O/W microemulsion.HPLC was used to determine chloramphenicol palmitate on C₁₈ column（250 mm×4.60 mm,5 μm）,the mobile phase consisted of methanol,the flow rate was 1.0 ml·min^-1,the column temperature of 25 ℃,the injection volume was 20 μl.RESULTS Chloramphenicol palmitate in phosphatidylcholine O/W microemulsions were prepared and the method of determination was developed.CONCLUSION Chloramphenicol palmitate can be used in O/W phosphatidycholine microemulsions.HPLC can be used to determine.更多还原