【摘要】 目的:研究止喘胶囊中淫羊藿苷和蛇床子素含量测定的方法。方法:采用Kromasil C18色谱柱(250 mm×4.6mm,5μm),流动相为甲醇-1.25%醋酸的梯度洗脱,流速1.0 mL/min,室温,利用二极管阵列检测器,在270 nm和322 nm两个波长下同时测定止喘胶囊中淫羊藿苷和蛇床子素的含量。结果:该方法分离效果好,精密度高,加样回收率令人满意。淫羊藿苷在0.228~2.28μg间有良好的线性关系,r=0.999 7;蛇床子素在0.430~4.300μg之间有良好的线性关系,r=0.999 3。结论:HPLC法可用于止喘胶囊的质量控制。更多还原

【Abstract】 Objective: To establish a HPLC method for determination of icariin and cnidiadin in Zhichuan capsule.（Methods: A） precise HPLC method was developed and validated for the simultaneous determination of icariin and cnidiadin on Kromasil C₁₈ column（250 mm×4.6 mm,5 μm）.Methanol and 1.25% acetic acid water was used as mobile phase with gra-（dient） elution at a flow rate of 1.0 mL/min.The assay of icariin and cnidiadin was quantified at the temperature of 25℃,with diode（array） detector,at the wavelength of 270 nm and 322 nm,respectively.Results: Good separation,high precision and a satisfactory recovery were demonstrated by the method,which was found to be linear over the range of 0.228-2.28 μg for（icariin）（r=（0.999 7）,） and 0.430-4.300 μg for cnidiadin（r= 0.999 3）.Conclusion: The method is suitable for quality control of Zhichuan capsule.更多还原