【摘要】 研究固定化面包酵母FD-12对不同水/有机溶剂两相体系的耐受性及不对称合成(S)-2-辛醇的还原反应。结果表明,固定化面包酵母FD-12在水/正十二烷两相体系中的耐受性最好,糖代谢活性保留值可达99.59%。不同的水/有机溶剂两相体系对生物转化影响较大。对反应条件进行优化,在水/正十二烷相体积比为20/5,固定化细胞用量为15.0 g,初始底物2-辛酮浓度为200 mmol/L的条件下转化96 h,(S)-2-辛醇产物得率可达29.59%,ee(S)值达98.81%。更多还原

【Abstract】 Immobilized baker’s yeast FD-12 was employed to the asymmetric synthesis of(S)-2-octanol in the different water/organic solvent biphasic systems.The results showed that the maximal tolerance of immobilized FD12 was observed in water/n-dodecane biphasic system with the value of metabolic activity retention being 99.59%.The experimental data also indicated that the reaction was strongly influenced by the organic solvents selected in the biphasic systems.Under the optimal conditions,that is,(15()g) immobilized cells in water/n-dodecane biphasic system with the Vaq/Vorg being 20/5,by adding 200()mmol/L 2-octanone,a yield of 29.59% and an e.e.of 98.81% of(S)-2-octanol were obtained in(96()h.)更多还原