【摘要】 用裂殖酵母作为生物催化剂,催化苯甲醛与丙酮酸合成L-苯基乙酰基甲醇(L-PAC),后者为合成L-麻黄素等多种药物的前体,转化反应的最佳温度为28℃,pH6.0,时间16 h,苯甲醛用量160~200 mmol/L,丙酮酸120~180 mmol/L,L-PAC产量可达15 g/L。更多还原

【Abstract】 The yeast-mediated biotransformation of benzaldehyde and pyruvic acid to L-Phenylacetylcarbinol(L-PAC),a key intermediate for L-ephedrine synthesis,occurs in citrate buffer(pH6.0) containing 10% free yeast cells aged of 18h at temperature of 28℃.The highest L-PAC concentration of 15()g/L was achieved by adding 150mmol/L benzaldehyde with 180mmol/L pyruvic acid in batches.更多还原